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Functions of the nuclear ubiquitin proteasome system regulated by N-myristoylation

Research Project

Project/Area Number 25840039
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionYokohama City University

Principal Investigator

KIMURA Ayuko  横浜市立大学, 生命医科学研究科, 特任助教 (50553616)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsユビキチン / ミリストイル化 / プロテアソーム / プロテオミクス / Rpt2 / 翻訳後修飾 / 核 / 細胞内局在 / ユビキチン化
Outline of Final Research Achievements

N-Myristoylation is expected to control the compartmentalized proteolysis within the cells via dynamic regulation of the nucleo-cytoplasmic localization of proteasome. To obtain the comprehensive view on the substrates and physiological functions of this control system, ubiquitin proteome analysis was performed using the yeast strains with or without mutation on the N-myristoylation site of proteasome subunit Rpt2. The ubiquitination levels of the two heat shock proteins involved in the nuclear transport of proteins as well as those of some possible nucleo-cytoplasmic proteins, which are involved in the stress response and metabolism, were significantly upregulated in the mutant strains. These results indicate that the N-myristoylation of proteasome is implicated in the stress response by controlled proteolysis of a part of cytoplasmic proteins within the nucleus.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Mass spectrometric analysis of the phosphorylation levels of the SWI/SNF chromatin remodeling/tumor suppressor proteins ARID1A and Brg1 in ovarian clear cell adenocarcinoma cell lines.2014

    • Author(s)
      Kimura, A., Arakawa, N., and Hirano, H.
    • Journal Title

      J Proteome Res.

      Volume: 13(11) Issue: 11 Pages: 4959-4969

    • DOI

      10.1021/pr500470h

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 卵巣明細胞腺癌細胞株特異的なクロマチン再構成因子Brg1のリン酸化レベル低下2014

    • Author(s)
      木村 鮎子, 荒川 憲昭, 平野 久
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] MRM法による卵巣明細胞腺癌細胞株特異的なSWI/SNFクロマチン再構成複合体因子Brg1のリン酸化レベル低下の解析2014

    • Author(s)
      木村 鮎子, 荒川 憲昭, 平野 久
    • Organizer
      日本プロテオーム学会第12回大会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification and Characterization of the Posttranslational Modifications of Yeast 26S Proteasome2013

    • Author(s)
      Ayuko Kimura, Yu Kato, Yoichi Kurata, Yayoi Kimura, Hisashi Hirano
    • Organizer
      HUPO 12th Annual World Congress
    • Place of Presentation
      Pacifico Yokohama, Japan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 医学のあゆみ 9章. 「疾患研究における翻訳後修飾の解析」2014

    • Author(s)
      木村 鮎子、平野 久
    • Total Pages
      1034
    • Publisher
      医歯薬出版
    • Related Report
      2014 Annual Research Report
  • [Book] プロテオミクス辞典2013

    • Author(s)
      日本プロテオーム学会編
    • Total Pages
      135
    • Publisher
      講談社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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