Research Project
Grant-in-Aid for Young Scientists (B)
T cell development depends on appropriate signals mediated by T cell receptors and many other receptors. Sphingolipids (gangliosides and sphingomyelin) are thought to form a platform (lipid raft) for receptor-mediated signaling.We determined the expression profiles of gangliosides during T cell development. Ganglioside levels were greatly increased during the DN3 stage, which was coincidentally with TCRb chain expression. To determine the role of gangliosides in T cell development we prepared GM3S and GM2/GD2S double deficient mice (DKO mice) which don’t express any ganglioside species. DKO mice exhibited abnormal thymic development and greatly reduced in thymocytes. T cell development in DKO mice was arrested at the DN3 stage where TCRb chain gene arrangement occurs. These results suggest unique rafts are formed in the plasma membrane during T cell development and these rafts provide the appropriate distinct intracellular signaling events for successful differentiation.
All 2015 2014 2013
All Journal Article (4 results) (of which Peer Reviewed: 4 results, Open Access: 1 results, Acknowledgement Compliant: 1 results) Presentation (8 results) Book (2 results)
Biochim Biophys Acta.
Volume: 1851 Issue: 1 Pages: 98-106
10.1016/j.bbalip.2014.07.013
Glycobiology
Volume: 25 Issue: 3 Pages: 303-318
10.1093/glycob/cwu112
Cell Mol Life Sci
Volume: 70 Issue: 17 Pages: 2000-2008
10.1007/s00018-012-1208-x
Trends in Glycoscience and Glycotechnology
Volume: 25 Issue: 144 Pages: 159-169
10.4052/tigg.25.159
130004841373
