| Project/Area Number |
25840041
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NISHIDA Kazumichi 東京大学, 理学(系)研究科(研究院), 助教 (10598436)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | piRNA生合成 / PIWIタンパク質 / RNAヘリカーゼタンパク質 / 生殖細胞 / カイコ / piRNA / トランスポゾン / BmVasa |
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| Outline of Final Research Achievements |
piRNAs associate with PIWI proteins and silence selfish transposable elements. piRNA biogenesis consists of two sequential steps; primary piRNA processing and the Ping-Pong cycle. However, piRNA biogenesis remains elusive. We aimed to elucidate about piRNA pathway.
A Bombyx mori germ cell line, BmN4, is an excellent tool to investigate the molecular pathways of piRNA biogenesis. BmN4 expresses two PIWI proteins (Siwi and BmAgo3). We show that Siwi binds with BmVasa. BmVasa is DEAD-box RNA helicase protein. A lack of BmVasa abolishes the Ping-Pong cycle. Also, Siwi is associated with BmSpn-E. BmSpn-E, unlike BmVasa, is necessary for primary piRNA production. We propose a new model for piRNA biogenesis, where the BmSpn-E function in primary piRNA processing, whereas BmVasa is important for the Ping-Pong cycle.
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