Novel carrier-mediated protein transport from the Golgi complex to the cell surface

• Project/Area Number: 25840042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: WAKANA Yuichi 東京薬科大学, 生命科学部, 助教 (90635187)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 小胞輸送 / 輸送キャリアー / ゴルジ体 / 細胞膜 / 小胞体 / キネシン / 膜接触部位 / 微小管 / 細胞分裂

Outline of Final Research Achievements

Previously, we have identified a new class of Golgi-to-cell surface carriers, CARTS. Here we report that kinesin-5/Eg5 is involved in migration of CARTS on microtubules. Eg5 is well known for its function in bipolar spindle assembly in mitotic cells and our results have revealed its new role in non-mitotic cells. We have also shown that CARTS biogenesis requires lipid transfer at the endoplasmic reticulum (ER)-Golgi contact sites, which suggests a direct role of the ER in regulating transport carrier formation at the Golgi complex.

Research Products

• [Journal Article] MAMの新たな機能と関連疾患 (2014)
  • Author(s): 多賀谷光男, 若菜裕一, 川端美緒
  • Journal Title: 細胞工学 Volume: 33 Pages: 436-442
  • NAID: 40020031737
• [Journal Article] Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface. (2013)
  • Author(s): Wakana, Y., Villeneuve, J., van Galen, J., Cruz-Garcia, D., Tagaya, M., and Malhotra, V.
  • Journal Title: J. Cell Biol. Volume: 202 Issue: 2 Pages: 241-250
  • DOI: 10.1083/jcb.201303163
  • Peer Reviewed
• [Journal Article] A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex. (2013)
  • Author(s): A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.
  • Journal Title: Mol Biol Cell Volume: 24 Issue: 18 Pages: 2907-2917
  • DOI: 10.1091/mbc.e13-01-0014
  • Peer Reviewed
• [Presentation] Roles of endoplasmic reticulum-Golgi contacts in transport carrier biogenesis from the trans-Golgi network (2015)
  • Author(s): Yuichi Wakana, Richika Kotake, Nanako Oyama, and Mitsuo Tagaya
  • Organizer: 第67回日本細胞生物学会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-30 – 2015-07-02
  • Invited
• [Presentation] VAPが仲介する小胞体-トランスゴルジ網（TGN）接触部位における脂質輸送は、TGNからの輸送小胞形成に必要である (2014)
  • Author(s): 若菜裕一、小竹莉愛、大山南菜子、多賀谷光男
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 脂質ホスファターゼSac1はVAPと相互作用し、トランスゴルジ網に近接した小胞体サブドメインに共局在する (2014)
  • Author(s): 大山南菜子、若菜裕一、小竹莉愛、多賀谷光男
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] キネシン－５/Eg5はトランスゴルジ網から細胞膜へのCARTSの輸送に関与する (2013)
  • Author(s): Wakana Y, Villeneuve J, van Galen J, Cruz-Garcia D, Tagaya M, Malhotra V
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜