| Project/Area Number |
25840044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hokkaido University |
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Principal Investigator |
OTOMO Kohei 北海道大学, 電子科学研究所, 特任助教 (40547204)
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| Co-Investigator(Renkei-kenkyūsha) |
NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
WATANABE Masakatsu 光産業創成大学院大学, 特任教授 (40144226)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 多光子顕微鏡 / 超解像顕微鏡 / 光遺伝学 / 多光子顕微鏡法 / 超解像顕微鏡法 / 多点走査型顕微鏡法 / 多点走査型共焦点顕微鏡 / 光刺激 |
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| Outline of Final Research Achievements |
The research goal was to develop new multi-photon excitation fluorescence microscopy systems which are beneficial for optogenetics studies. Specifically, we aimed to develop a microscopy system that enables light activation control of cyclic adenosine monophosphate (cAMP) level in the single cell locating at the deep region of a live specimen, and visualization of the changes of cAMP level at the high spatiotemporal resolution. By utilizing a multipoint laser scanning method, development of a microscopy system that enables the high temporal resolution multi-photon excitation fluorescence imaging and the locally multi-photon activation was achieved. Furthermore, by utilizing a stimulated emission depletion process, a new two-photon excitation super-resolution microscopy technique was developed. At present, we approach to locally cAMP control in a live specimen by use of developed microscopy systems.
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