| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Phototropins (phots) are blue-light dependent kinases in higher plants as well as in green algae. They contain two LOV domains (LOV1 and LOV2) as light sensing modules. In this study, we investigated the reaction dynamics of full-length phot and its shorter fragments from Chlamydomonas reinhardtii (Cr-phot) to understand the mechanism of kinase activation by the transient grating (TG) method.
We have detected global reactions in milliseconds time scale as a decrease of diffusion coefficient (D). The rate constant of the D change depended on the protein concentration linearly, indicating that the reaction was bimolecular reaction (dimerization). This reaction was mediated by the LOV1 domain and the contribution of the LOV2 domain to the D change was very minor. These findings are different from those of phot from Arabidopsis, which represents the diversity of phot among organisms.
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