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Molecular mechanism of kinase activation of light sensor protein phototropin

Research Project

Project/Area Number 25840047
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

NAKASONE Yusuke  京都大学, 理学(系)研究科(研究院), 助教 (00613019)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsphototropin / LOVドメイン / 過渡回折格子法 / 蛋白質反応 / 光センサータンパク質 / 光センサー蛋白質
Outline of Final Research Achievements

Phototropins (phots) are blue-light dependent kinases in higher plants as well as in green algae. They contain two LOV domains (LOV1 and LOV2) as light sensing modules. In this study, we investigated the reaction dynamics of full-length phot and its shorter fragments from Chlamydomonas reinhardtii (Cr-phot) to understand the mechanism of kinase activation by the transient grating (TG) method.
We have detected global reactions in milliseconds time scale as a decrease of diffusion coefficient (D). The rate constant of the D change depended on the protein concentration linearly, indicating that the reaction was bimolecular reaction (dimerization). This reaction was mediated by the LOV1 domain and the contribution of the LOV2 domain to the D change was very minor. These findings are different from those of phot from Arabidopsis, which represents the diversity of phot among organisms.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Photo-Induced Oligomerization of Arabidopsis Thaliana Phototropin 2 LOV12014

    • Author(s)
      Y. Nakasone, Y. Kawaguchi, S-G. Kong, M. Wada, M. Terazima
    • Journal Title

      J.Phys.Chem.B

      Volume: 118 Issue: 49 Pages: 14314-14325

    • DOI

      10.1021/jp509448b

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photochemistry of Arabidopsis phototropin 1 LOV1: transient tetramerization2013

    • Author(s)
      Y. Nakasone, K. Zikihara, S. Tokutomi,M. Terazima
    • Journal Title

      Photochem.Photobiol.Sci.

      Volume: 12 Issue: 7 Pages: 1171-1179

    • DOI

      10.1039/c3pp50047k

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamics of the Amino-Terminal and Carboxyl-Terminal Helices of Arabidopsis Phototropin 1 LOV2 Studied by the Transient Grating2013

    • Author(s)
      K.Takeda, Y.Nakasone, K.Zikihara, S.Tokutomi, M. Terazima
    • Journal Title

      J.Phys.Chem.B,

      Volume: 117 Issue: 49 Pages: 15606-15613

    • DOI

      10.1021/jp406109j

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] TIME-RESOLVED STUDY ON PHOTOREACTION DYNAMICS OF FULL-LENGTH PHOTOTROIN FROM GREEN ALGAE2015

    • Author(s)
      中曽根祐介
    • Organizer
      新学術領域研究「動的秩序と機能」第3回国際シンポジウム
    • Place of Presentation
      三重
    • Year and Date
      2015-01-10 – 2015-01-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] TIME-RESOLVED STUDY ON PHOTOREACTION DYNAMICS OF FULL-LENGTH PHOTOTROIN FROM GREEN ALGAE2014

    • Author(s)
      中曽根祐介
    • Organizer
      16th International Conference on Retinal Proteins
    • Place of Presentation
      滋賀
    • Year and Date
      2014-10-05 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Time-resolved study on transient dimerization of full-length phototropin2014

    • Author(s)
      中曽根祐介
    • Organizer
      The 2nd international symposium on dynamical ordering of biomolecular systerms for creation of integrated functions
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Thermodynamic measurement of the structural fluctuation in the signaling state of phototropin2013

    • Author(s)
      中曽根祐介
    • Organizer
      33rd International conference on solution chemistry
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Photochemistry of full-length phototropin from green algae2013

    • Author(s)
      中曽根祐介
    • Organizer
      第51回生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Time-resolved study on photoreaction dynamics of full-length phototropin from green algae2013

    • Author(s)
      中曽根祐介
    • Organizer
      The 6th Asia & Oceania conference on photobiology
    • Place of Presentation
      シドニー
    • Related Report
      2013 Research-status Report
  • [Remarks] 光物理化学研究室ホームページ

    • URL

      http://kuchem.kyoto-u.ac.jp/hikari/index.html

    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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