Interfacial analysis using artificial exo/endocytotic systems and development of target-specific secretion/uptake systems
Project/Area Number |
25840051
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biophysics
|
Research Institution | Teikyo University (2014) Nagoya City University (2013) |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 人工細胞 / 開口放出 / リポソーム / エキソサイトーシス / エンドサイトーシス |
Outline of Final Research Achievements |
Previously, we developed an artificial exocytotic system that secretes intravesicular contents upon Ca2+ influx in GUV. In this study, we cleared the effect of osmolality gap between the inside and the outside of GUV and lipid composition of GUV on exocytotic release. We also found that Ca2+ influx into GUVs was induced by amyloid beta, suggesting that our artificial exocytotic system could induce exocytosis-like secretion by Ca2+ elevation in GUV. These results will lead to the development of artificial exocytotic systems which release amyloid-degrading enzymes by recognizing amyloid beta.
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Report
(3 results)
Research Products
(15 results)