| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Previously, we developed an artificial exocytotic system that secretes intravesicular contents upon Ca2+ influx in GUV. In this study, we cleared the effect of osmolality gap between the inside and the outside of GUV and lipid composition of GUV on exocytotic release. We also found that Ca2+ influx into GUVs was induced by amyloid beta, suggesting that our artificial exocytotic system could induce exocytosis-like secretion by Ca2+ elevation in GUV. These results will lead to the development of artificial exocytotic systems which release amyloid-degrading enzymes by recognizing amyloid beta.
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