| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
About macro protein complexes, we developed a new method for directly-extracted ribosomes from unicellular organism (E.coli) and higher animal cells (HEK293: human cultured cell) by mass spectrometry and H/D exchange, to progress the function analysis in the cell through the structural analysis. We succeeded in development of a method, which detects structural changes of many ribosomal proteins from sonicated cell extracts under 1mg cells, by rough purification and optimization of MALDI matrix. This developed method suggested a possibility for the directly detection of structural change from individual target without the gene cloning and over expressions.
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