semi-in vivo analysis on structural dynamics of ribosome complex by mass spectrometry
| Project/Area Number |
25840052
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Suntory Foundation for Life Sciences (2014-2015)
Keio University (2013) |
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Principal Investigator |
Yamamoto Tatsuya 公益財団法人サントリー生命科学財団, 統合生体分子機能研究部, 研究員 (70437573)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リボソーム / 構造機能相関 / 細胞 |
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| Outline of Final Research Achievements |
About macro protein complexes, we developed a new method for directly-extracted ribosomes from unicellular organism (E.coli) and higher animal cells (HEK293: human cultured cell) by mass spectrometry and H/D exchange, to progress the function analysis in the cell through the structural analysis. We succeeded in development of a method, which detects structural changes of many ribosomal proteins from sonicated cell extracts under 1mg cells, by rough purification and optimization of MALDI matrix. This developed method suggested a possibility for the directly detection of structural change from individual target without the gene cloning and over expressions.
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Report
(4 results)
Research Products
(1 results)
