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Elucidation of operation mechanism of rotary molecular motors by comparison with the mechanisms of F1 and V1-ATPases.

Research Project

Project/Area Number 25840053
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionChuo University

Principal Investigator

UENO Hiroshi  中央大学, 理工学部, 助教 (10546592)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords1分子計測 / 分子モーター
Outline of Final Research Achievements

We have succeeded in analyzing the ATP-driven rotation of Enterococcus hirae V1-ATPase at single-molecule level for the first time. The detailed analysis of the rotation and the comparison with V1-ATPase from another species revealed the common rotary mechanism of V1-ATPase. Furthermore, we determined the rotary angle at which the elementary reactions of ATP hydrolysis occur. These results lead to a deeper understanding of operation mechanism of rotary molecular motors.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analysis2015

    • Author(s)
      Iino R, Minagawa Y, Ueno H, Suzuki K, and Murata T
    • Journal Title

      Current Opinion in Structural Biology

      Volume: 31 Pages: 49-56

    • DOI

      10.1016/j.sbi.2015.02.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Torque generation of Enterococcus hirae V-ATPase2014

    • Author(s)
      Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 45 Pages: 31212-31223

    • DOI

      10.1074/jbc.m114.598177

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular structure and rotary dynamics of Enterococcus hirae V1-ATPase2014

    • Author(s)
      Iino R, Minagawa Y, Ueno H, Hara M, and Murata T
    • Journal Title

      IUBMB Life

      Volume: 66 Issue: 9 Pages: 624-630

    • DOI

      10.1002/iub.1311

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic analyses of nucleotide binding to an isolated monomeric β subunit and the α3β3γ subcomplex of F1-ATPase.2013

    • Author(s)
      Kikuchi, Y., Naka, Y., Osakabe, H., Okamoto, T., Masaike, T., Ueno, H., Toyabe, S., Muneyuki, E.
    • Journal Title

      Biophysical Journal

      Volume: 105 Issue: 11 Pages: 2541-2548

    • DOI

      10.1016/j.bpj.2013.10.018

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase2013

    • Author(s)
      Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
    • Journal Title

      J.Biol.Chem

      Volume: 288 Issue: 45 Pages: 32700-32707

    • DOI

      10.1074/jbc.m113.506329

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase2013

    • Author(s)
      Watanabe R, Hayashi K, Ueno H and Noji H
    • Journal Title

      Biophys. J

      Volume: 105 Issue: 10 Pages: 2385-2391

    • DOI

      10.1016/j.bpj.2013.09.050

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Common evolutionary origin for the rotor domain of rotary ATPases and Flagellar protein export apparatus.2013

    • Author(s)
      Kishikawa, J., et al
    • Journal Title

      PLoS One

      Volume: 8 Issue: 5 Pages: 64692013-64692013

    • DOI

      10.1371/journal.pone.0064695

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 腸内連鎖球菌由来のV-ATPaseの1分子回転計測2015

    • Author(s)
      上野博史、皆川慶嘉、宗行英朗、野地博行、村田武士、飯野亮太
    • Organizer
      分子研研究会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2015-04-20 – 2015-04-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナトリウムを輸送するV-ATPaseの1分子回転観察2014

    • Author(s)
      上野博史
    • Organizer
      第6回膜輸送体研究会
    • Place of Presentation
      ニュー富良野ホテル
    • Year and Date
      2014-12-26 – 2014-12-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase2014

    • Author(s)
      上野博史、皆川慶嘉、原舞雪、山登一郎、野地博行、村田武士、飯野亮太、宗行英朗
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] The role of amino acid residues located at the catalytic site in the rotation of Enterococcus hirae V1-ATPase2014

    • Author(s)
      皆川慶嘉、上野博史、原舞雪、野地博行、村田武士、飯野亮太
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interactions between Rotor and Stator in Enterococcus hirae V-ATPase2014

    • Author(s)
      Ueno H, Minagawa Y, Hara M, Noji H, Murata T, Iino R, Muneyuki E
    • Organizer
      Tokyo ATPase Workshop
    • Place of Presentation
      東京大学弥生キャンパス武田ホール
    • Year and Date
      2014-06-02 – 2014-06-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase2014

    • Author(s)
      Minagawa Y, Ueno H, Hara M, Noji H, Murata T, Iino R
    • Organizer
      Tokyo ATPase Workshop
    • Place of Presentation
      東京大学弥生キャンパス武田ホール
    • Year and Date
      2014-06-02 – 2014-06-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Direct observation of the rotation of V1-ATPase from Enterococcus hirae and its torque2013

    • Author(s)
      上野 博史, 皆川 慶嘉, 山登 一郎, 村田 武志, 飯野 亮太, 宗行 英朗
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] The relationship between F1-ATPase P-loop mutants and Pi release2013

    • Author(s)
      吉田 光, 伊藤 亜由美, 伊藤 丈太郎, ,政池 知子, 西坂 崇之, 鳥谷部 祥一, 上野 博史, 宗行 英朗
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Comparison of the nucleotide binding to the isolated β subunit and the F1-ATPase using the Sopped-Flow method2013

    • Author(s)
      長埜 陸, 小原 清志, 政池 知子, 上野 博史, 宗行 英朗
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Basic properties of rotary dynamics of Enterococcus hirae V1-ATPAse motor protein2013

    • Author(s)
      皆川 慶嘉, 上野 博史, 石塚 芳子, 大沢 登, 寺田 貴帆, 白水 美香子, 横山 茂之, 野地 博行, 村田 武士, 飯野 亮太
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Book] FoF1モーター 「DOJIN BIOSCIENCE SERIES 17 1分子生物学」2014

    • Author(s)
      上野博史, 鳥谷部祥一, 宗行英朗
    • Total Pages
      13
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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