Elucidation of operation mechanism of rotary molecular motors by comparison with the mechanisms of F1 and V1-ATPases.

• Project/Area Number: 25840053
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Chuo University
• Principal Investigator: UENO Hiroshi 中央大学, 理工学部, 助教 (10546592)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 1分子計測 / 分子モーター

Outline of Final Research Achievements

We have succeeded in analyzing the ATP-driven rotation of Enterococcus hirae V1-ATPase at single-molecule level for the first time. The detailed analysis of the rotation and the comparison with V1-ATPase from another species revealed the common rotary mechanism of V1-ATPase. Furthermore, we determined the rotary angle at which the elementary reactions of ATP hydrolysis occur. These results lead to a deeper understanding of operation mechanism of rotary molecular motors.

Research Products

• [Journal Article] Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analysis (2015)
  • Author(s): Iino R, Minagawa Y, Ueno H, Suzuki K, and Murata T
  • Journal Title: Current Opinion in Structural Biology Volume: 31 Pages: 49-56
  • DOI: 10.1016/j.sbi.2015.02.013
  • Peer Reviewed
• [Journal Article] Torque generation of Enterococcus hirae V-ATPase (2014)
  • Author(s): Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 45 Pages: 31212-31223
  • DOI: 10.1074/jbc.m114.598177
  • Peer Reviewed / Open Access
• [Journal Article] Molecular structure and rotary dynamics of Enterococcus hirae V1-ATPase (2014)
  • Author(s): Iino R, Minagawa Y, Ueno H, Hara M, and Murata T
  • Journal Title: IUBMB Life Volume: 66 Issue: 9 Pages: 624-630
  • DOI: 10.1002/iub.1311
  • Peer Reviewed
• [Journal Article] Thermodynamic analyses of nucleotide binding to an isolated monomeric β subunit and the α3β3γ subcomplex of F1-ATPase. (2013)
  • Author(s): Kikuchi, Y., Naka, Y., Osakabe, H., Okamoto, T., Masaike, T., Ueno, H., Toyabe, S., Muneyuki, E.
  • Journal Title: Biophysical Journal Volume: 105 Issue: 11 Pages: 2541-2548
  • DOI: 10.1016/j.bpj.2013.10.018
  • Peer Reviewed
• [Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase (2013)
  • Author(s): Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
  • Journal Title: J.Biol.Chem Volume: 288 Issue: 45 Pages: 32700-32707
  • DOI: 10.1074/jbc.m113.506329
  • Peer Reviewed
• [Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase (2013)
  • Author(s): Watanabe R, Hayashi K, Ueno H and Noji H
  • Journal Title: Biophys. J Volume: 105 Issue: 10 Pages: 2385-2391
  • DOI: 10.1016/j.bpj.2013.09.050
  • Peer Reviewed
• [Journal Article] Common evolutionary origin for the rotor domain of rotary ATPases and Flagellar protein export apparatus. (2013)
  • Author(s): Kishikawa, J., et al
  • Journal Title: PLoS One Volume: 8 Issue: 5 Pages: 64692013-64692013
  • DOI: 10.1371/journal.pone.0064695
  • Peer Reviewed
• [Presentation] 腸内連鎖球菌由来のV-ATPaseの1分子回転計測 (2015)
  • Author(s): 上野博史、皆川慶嘉、宗行英朗、野地博行、村田武士、飯野亮太
  • Organizer: 分子研研究会
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2015-04-20 – 2015-04-21
• [Presentation] ナトリウムを輸送するV-ATPaseの1分子回転観察 (2014)
  • Author(s): 上野博史
  • Organizer: 第6回膜輸送体研究会
  • Place of Presentation: ニュー富良野ホテル
  • Year and Date: 2014-12-26 – 2014-12-28
• [Presentation] Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase (2014)
  • Author(s): 上野博史、皆川慶嘉、原舞雪、山登一郎、野地博行、村田武士、飯野亮太、宗行英朗
  • Organizer: 第52回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] The role of amino acid residues located at the catalytic site in the rotation of Enterococcus hirae V1-ATPase (2014)
  • Author(s): 皆川慶嘉、上野博史、原舞雪、野地博行、村田武士、飯野亮太
  • Organizer: 第52回日本生物物理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Interactions between Rotor and Stator in Enterococcus hirae V-ATPase (2014)
  • Author(s): Ueno H, Minagawa Y, Hara M, Noji H, Murata T, Iino R, Muneyuki E
  • Organizer: Tokyo ATPase Workshop
  • Place of Presentation: 東京大学弥生キャンパス武田ホール
  • Year and Date: 2014-06-02 – 2014-06-03
• [Presentation] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase (2014)
  • Author(s): Minagawa Y, Ueno H, Hara M, Noji H, Murata T, Iino R
  • Organizer: Tokyo ATPase Workshop
  • Place of Presentation: 東京大学弥生キャンパス武田ホール
  • Year and Date: 2014-06-02 – 2014-06-03
• [Presentation] Direct observation of the rotation of V1-ATPase from Enterococcus hirae and its torque (2013)
  • Author(s): 上野 博史, 皆川 慶嘉, 山登 一郎, 村田 武志, 飯野 亮太, 宗行 英朗
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] The relationship between F1-ATPase P-loop mutants and Pi release (2013)
  • Author(s): 吉田 光, 伊藤 亜由美, 伊藤 丈太郎, ,政池 知子, 西坂 崇之, 鳥谷部 祥一, 上野 博史, 宗行 英朗
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Comparison of the nucleotide binding to the isolated β subunit and the F1-ATPase using the Sopped-Flow method (2013)
  • Author(s): 長埜 陸, 小原 清志, 政池 知子, 上野 博史, 宗行 英朗
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Basic properties of rotary dynamics of Enterococcus hirae V1-ATPAse motor protein (2013)
  • Author(s): 皆川 慶嘉, 上野 博史, 石塚 芳子, 大沢 登, 寺田 貴帆, 白水 美香子, 横山 茂之, 野地 博行, 村田 武士, 飯野 亮太
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Book] FoF1モーター 「DOJIN BIOSCIENCE SERIES 17 1分子生物学」 (2014)
  • Author(s): 上野博史, 鳥谷部祥一, 宗行英朗
  • Total Pages: 13
  • Publisher: 化学同人