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Detection of structural polymorphism in actin inside a cell and elucidation of its physiological meaning.

Research Project

Project/Area Number 25840057
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionMiyakonojo National College of Technology

Principal Investigator

NOGUCHI Taro  都城工業高等専門学校, 物質工学科, 講師 (90615866)

Co-Investigator(Renkei-kenkyūsha) UYEDA Taro  産業技術総合研究所, セルメカニクス研究グループ (90356551)
NAGASAKI Akira  産業技術総合研究所, セルメカニクス研究グループ (30392640)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsアクチン / 構造
Outline of Final Research Achievements

Structural change of actin is important for cell functions. However, such polymorphism of actin has not been detected in cells, and its physiological relevance is unclear. In this study, to detect polymorphism of actin in cell, intramolecular FRET system was used.
Actin with the FRET probe was introduced into PtK2 cells using electroporator optimized for adherent cells, and were observed by confocal microscopy. The FRET ratio (intensities of acceptor/donor) in PtK2 cells differed locally, indicating that actin structure in cells is also variable.This revealed that actin subunits in cell take multiple conformations, and involved in cell functions.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] The Role of Structural Dynamics of Actin in Class-Specific Myosin Motility2015

    • Author(s)
      Noguchi, T.Q.P., Morimatsu, M., Iwane, A.H., Yanagida, T., Uyeda, T.Q.P.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 5 Pages: e0126262-e0126262

    • DOI

      10.1371/journal.pone.0126262

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Structural polymorphism of actin detected by intramolecular FRET in vivo and in vitro2014

    • Author(s)
      Taro Q.P. Noguchi, Mio Okazaki, Saku Kijima, Masatoshi Morimatsu, Akira Nagasaki, Yoshiaki Iwadate, Toshio Yanagida and Taro Q.P. Uyeda
    • Organizer
      ASCB annual meeting
    • Place of Presentation
      Philadelphia USA
    • Year and Date
      2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] FRET プローブアクチンを用いた細胞内におけるアクチンの構造多型の検出2014

    • Author(s)
      Mio Okazaki, Saku Kijima, Yoshiaki Iwadate, Taro Q.P Uyeda, Taro Q.P Noguchi
    • Organizer
      第52回 日本生物物理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] FRETプローブactinによるアクチンの構造変化検出

    • Author(s)
      児玉裕紀、貴嶋 紗久、野口太郎
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] ヌクレオチド状態の変化を伴わないアクチンフィラメントのダイナミックスタビリティー

    • Author(s)
      五十嵐圭、野口太郎
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Microtubule-associated protein 4-mediated bundle formation of microtubules and actin filaments

    • Author(s)
      齊藤翔馬, 倉本歩, 槇原光, 山崎剛, 野口太郎, 小谷享, 徳楽清孝
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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