| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Aggregation of Dictyostelium discoideum is mediated by chemotaxis towards propagating waves of chemoattractant cAMP. Because the direction of chemoattractant gradient reversed during the wave passage, how unidirectional migration is achieved remains unresolved. By precise microfluidics emulation of the dynamic chemoattractant field we demonstrate that, in Dictyostelium cells, directional migration as well as activation of small guanosine triphosphatase Ras at the leading edge is suppressed when the chemoattractant concentration is decreasing over time. The ‘rectification’ of directional sensing occurs only at an intermediate range of wave speed and its optimum is at about a period of 6 minutes which is consistent with the wave period typically observed in aggregating cells. These results indicate that the ability of Dictyostelium cells to move only in the wavefront is closely associated with rectification of adaptive response combined with local activation and global inhibition.
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