Development of correlation microscopy between deep-etch EM and photoactivation localization microscopy. Application to study of membrane dynamics.

Research Project

Project/Area Number	25840071
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Cell biology
Research Institution	Kyoto University
Principal Investigator	YOSHIMURA Azumi 京都大学, 物質-細胞統合システム拠点, 研究員 (90467340)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	カベオラ / 電子顕微鏡 / 超解像度顕微鏡法 / correlation microscopy / 電子顕微鏡法
Outline of Final Research Achievements	A method of correlative imaging between DEEM and photo-activation localization microscopy (PALM) was developed in order to link the localization of the molecules and the topological information with higher efficiency and accuracy. Using this method, the relationship between curvature of caveolae, the plasma membrane invaginations, and their main component caveolin was examined. The correlated images show that caveolae of all curvatures contain caveolin cluster.

Report

(3 results)