The function of a novel ER modulating factor, Pecanex in Notch signaling

• Project/Area Number: 25840074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YAMAKAWA Tomoko 大阪大学, 理学(系)研究科(研究院), 助教 (20645402)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Notchシグナル / 小胞体 / ショウジョウバエ

Outline of Final Research Achievements

Notch signaling regulates many cell-fate decision in multi-cellular organism and its defects causes various diseases in human like cancer. pecanex (pcx) is a component of Notch signaling. My study revealed that Pcx is localized to the ER and required for the ER morphogenesis. To further understanding of pcx molecular function, I screened pcx modifiers and identified 6 candidate genes. I obtained 4 genes, which encodes known Notch signaling components, Nsf2 encoding an ATPase required for the vesicle fusion and jim lovell encoding a transcription factor. And I showed that these candidate genes genetically interact with pcx.

Research Products

• [Journal Article] A gain-of-function screen to identify genes that reduce lifespan in the adult of Drosophila melanogaster. (2014)
  • Author(s): Nakayama M, Ishibashi T, Ishikawa HO, Sato H, Usui T, Okuda T, Yashiro H, Ishikawa H, Taikou Y, Minami A, Kato K, Taki M, Aigaki T, Gunji W, Ohtsu M, Murakami Y, Tanuma S, Tsuboi A, Adachi M, Kuroda J, Sasamura T, Yamakawa T, Matsuno K,
  • Journal Title: BMC Genet. Volume: 16 Issue: 1
  • DOI: 10.1186/1471-2156-15-46
  • Peer Reviewed / Open Access
• [Presentation] The function of novel ER modulating factor, Pecanex in Notch signaling (2015)
  • Author(s): 山川智子
  • Organizer: 3rd Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 北京 (中国)
  • Year and Date: 2015-05-11 – 2015-05-14
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila (2014)
  • Author(s): 山川智子
  • Organizer: Notch研究会
  • Place of Presentation: 大阪大学 (大阪府、吹田市)
  • Year and Date: 2014-12-18
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila. (2014)
  • Author(s): 山川智子
  • Organizer: Gordon Conference
  • Place of Presentation: Bates College (USA)
  • Year and Date: 2014-07-19 – 2014-07-25
• [Presentation] The function of novel ER modulating factor, Pecanex in Notch signaling (2014)
  • Author(s): 山川智子
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂 (奈良県、奈良市)
  • Year and Date: 2014-06-11 – 2014-06-13
  • Invited
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila (2014)
  • Author(s): 山川智子
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: ウィンク愛知 (愛知県、名古屋市)
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Functions of a neurogenic gene, pecanex in Notch signaling (2014)
  • Author(s): Tomoko Yamakawa, Yu Atsumi, Shiori Kubo, Kenji Matsuno
  • Organizer: 55th Annual Drosophila Research Conference
  • Place of Presentation: San Diego, USA
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila (2013)
  • Author(s): Tomoko Yamakawa, Yu Atsumi, Shiori Kubo, Kenji Matsuno
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Notchシグナル伝達の構成因子、Pecanex (Pcx)の小胞体における機能の解析 (2013)
  • Author(s): 山川智子
  • Organizer: 日本発生生物学会2013年秋季シンポジウム
  • Place of Presentation: 神戸しあわせの村
• [Presentation] Functions of a neurogenic gene, pecanex in Notch signaling. (2013)
  • Author(s): Tomoko Yamakawa, Yu Atsumi, Shiori Kubo, Kenji Matsuno
  • Organizer: Notch meeting VII
  • Place of Presentation: Athens, Greece
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila (2013)
  • Author(s): Tomoko Yamakawa, Yu Atsumi, Shiori Kubo, Kenji Matsuno
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Identification of genetic modifiers that interact with pecanex, encoding a component of Notch signaling in Drosophila (2013)
  • Author(s): Tomoko Yamakawa, Yu Atsumi, Shiori Kubo, Kenji Matsuno
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 松江
• [Remarks] 大阪大学 細胞生物学研究室ホームページ
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/matsuno/index.html