Study of the mode of newt myocardial regeneration based on identification of the cell linage and functional analysis of the cell cycle related genes.
Project/Area Number |
25840086
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Tottori University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | イベリアトゲイモリ / 心臓再生 / 心筋細胞 / 細胞系譜 / トランスジェニック / ゲノム編集 / コンディショナルノックアウト / CRE-loxP |
Outline of Final Research Achievements |
Using Iberian ribbed newt that is useful a model animal for regeneration biology, we established a cell lineage tracing system by genetic labeling. We showed the pre-existing cardiomyocytes proliferated during cardiac regeneration in the newts. We reported that TALEN-mediated gene targeting and an infrared laser mediated local gene induction by IR-LEGO system in the P. waltl. Moreover, we prepared basic data for genome editing in the newts in order to establish conditional knock-out system that enable more detailed analysis in organ regeneration.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Application of local gene induction by infrared laser-mediated microscope and temperature stimulator to amphibian regeneration study.2015
Author(s)
Kawasumi, A., Hayashi, T., Kobayashi, T., Nagayama, C., Hayashi, S., Kamei, Y., Morishita, Y., T Takeuchi, T., Tamura, K., Yokoyama, H.
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Journal Title
Dev. Growth Differ.
Volume: 57
Issue: 9
Pages: 601-613
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014
Author(s)
Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
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Journal Title
Development, Growth and Differentiation
Volume: 56
Issue: 1
Pages: 115-121
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] TALEN-mediated genome editing is very useful in Iberian ribbed newts, an experimental model animal for regeneration.2014
Author(s)
Hayashi T., Sakamoto K., Sakuma T., Myouga A., Naoki Y., Inoue T., Kawaguchi E., Agata K., Yamamoto T., Takeuchi T.
Organizer
日本発生生物学会
Place of Presentation
愛知県名古屋市WINC AICHI
Year and Date
2014-05-27 – 2014-05-29
Related Report
Invited
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[Presentation] Molecular genetic system for regenerative studies using newts Iberian Ribbed Newts (Pleurodeles waltl).2014
Author(s)
Hayashi, T., Yokotani, N., Matsumoto, A., Myouga, A., Sakamoto, K., Inoue, T., Kawaguchi, E., Agata, K., Sakuma, T., Yamamoto, T., Takeuchi, T.
Organizer
CDB Symposium 2014
Place of Presentation
RIKEN CDB、神戸市
Related Report
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[Presentation] What molecule triggers re-proliferation of cardiomyocytes during newt heart regeneration?2013
Author(s)
Yokotani, N., Inoue, T., Kawaguchi, E., Agata, K., Takeuchi, T., Hayashi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
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[Presentation] Molecular genetic system for regenerative studies using newt.2013
Author(s)
Hayashi, T., Yokotani, N., Matsumoto, A., Myouga, A., Okamoto, M., Takeuchi, T.
Organizer
日本発生生物学会
Place of Presentation
くにびきメッセ 松江市
Related Report
Invited
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