Genetic mechanisms for the acquisition of cell-death resistances

• Project/Area Number: 25840088
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Gakushuin University
• Principal Investigator: Taniguchi Kiichiro 学習院大学, 理学部, 助教 (20554174)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: アポトーシス / アポトーシス耐性 / ショウジョウバエ / カスパーゼ / 細胞死耐性 / 附属腺 / 脂肪体 / 後腸 / 生殖器附属腺

Outline of Final Research Achievements

Apoptosis is a controlled cell death and a key process to eliminate malfunctioning and/or damaged cell for maintaining tissue homeostasis. Interestingly, the sensitivities to the stress-inducible apoptosis vary in different tissue. The sensitivities to the apoptosis is considered to be due to the acquisition of resistances to apoptosis. However, there are little studies that evidently demonstrates the physiological resistances to apoptosis in animal tissues. Here, we examined the physiological resistances to apoptosis in Drosophila tissues, male accessory gland, larval fat body and adult hindgut. In the results, we found that suppressed transcription of Dcp-1, an effector Caspase, was associated with resistances to apoptosis commonly in male accessory gland, larval fat body and adult hindgut. We also found that the hindgut additionally acquired some kind of suppression control downstream of effector Caspases.

Research Products

• [Journal Article] GATAe regulates intestinal stem cell maintenance and differentiation in Drosophila adult midgut. (2016)
  • Author(s): Okumura, T., Takeda, K., Kuchiki, M., Akaishi, M., Taniguchi, K. and Adachi-Yamada, T.
  • Journal Title: Developmental Biology Volume: 410 Issue: 1 Pages: 24-35
  • DOI: 10.1016/j.ydbio.2015.12.017
  • Peer Reviewed
• [Journal Article] Class I myosins have overlapping and specialized functions in left-right asymmetric development in Drosophila (2015)
  • Author(s): Okumura T, Sasamura T, Inatomi M, Hozumi S, Nakamura M, Hatori R, Taniguchi K, Nakazawa N, Suzuki E, Maeda R, Yamakawa T, Matsuno K
  • Journal Title: Genetics Volume: 199 Issue: 4 Pages: 1183-1199
  • DOI: 10.1534/genetics.115.174698
  • Peer Reviewed / Open Access
• [Journal Article] Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells. (2014)
  • Author(s): Kiichiro Taniguchi, et al.
  • Journal Title: BMC Developmental Biology Volume: 20 Issue: 1 Pages: 46-46
  • DOI: 10.1186/s12861-014-0046-5
  • Peer Reviewed / Open Access
• [Journal Article] Left–right asymmetry is formed in individual cells by intrinsic cell chirality (2014)
  • Author(s): (4)Hatori, R., Ando, T., Sasamura, T., Nakazawa, N., Nakamura, M., Taniguchi, K., Hozumi, S., Kikuta, J., Ishii, M., Matsuno, K.
  • Journal Title: Mech. Dev. Volume: 133 Pages: 146-162
  • DOI: 10.1016/j.mod.2014.04.002
  • Peer Reviewed
• [Journal Article] βν Integrin Inhibits Chronic and High Level Activation of JNK to Repress Senescence Phenotypes in Drosophila Adult Midgut (2014)
  • Author(s): Takashi Okumura, Koji Takeda, Kiichiro Taniguchi, Takashi Adachi-Yamada
  • Journal Title: PLoS ONE Volume: 9 Issue: 2 Pages: e89387-e89387
  • DOI: 10.1371/journal.pone.0089387
  • Peer Reviewed
• [Presentation] ショウジョウバエ組織における生理的アポトーシス耐性の解析 (2015)
  • Author(s): 谷口喜一郎, 中越英樹, 安達卓
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells (2014)
  • Author(s): 谷口 喜一郎、他
  • Organizer: 第11回日本ショウジョウバエ研究会
  • Place of Presentation: 金沢歌劇座
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Analysis of βν integrin mutant showing a premature aging phenotype in Drosophila adult midgut (2014)
  • Author(s): 奥村高志, 武田晃司, 谷口喜一郎, 安達卓
  • Organizer: 55rd Annual Drosophila Research Conference
  • Place of Presentation: サンディエゴ
• [Presentation] Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells (2014)
  • Author(s): 谷口喜一郎, 國領顯彦 , 今野貴夫, 南竜之介, 中越英樹, 安達卓
  • Organizer: 55rd Annual Drosophila Research Conference
  • Place of Presentation: サンディエゴ
• [Presentation] Mud/NuMAはショウジョウバエ雄生殖器附属腺において細胞二核化を制御する (2013)
  • Author(s): 谷口喜一郎, 國領顯彦 , 今野貴夫, 南竜之介, 中越英樹, 安達卓
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Remarks] 学習院大学・安達研究室
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/
• [Remarks] 学習院大学・安達研究室
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/index.html
• [Remarks] 学習院大学・安達研究室
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/index.html