The analysis of the molecular function of ROS signaling in neuroeplthelium development

• Project/Area Number: 25840090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Doshisha University
• Principal Investigator: Sakai Daisuke 同志社大学, 研究開発推進機構, 助教 (90632646)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 活性酸素種 / 脳発生 / 活性酸素 / 胚発生 / 呼吸代謝 / 活性酸素シグナル / 脳原基形成異常

Outline of Final Research Achievements

In this study, I investigated whether reactive oxygen species (ROS) function as signaling molecule on embryonic brain development. Consequently, I revealed that ROS were required for proliferation of neural progenitor cells and consequent neurogenesis. Furthermore, ROS were involved in cranial neural crest development. Tissue specific ROS generation appeared to be dependent on the activity of cellular metabolism, glycolysis, but independent on ROS-generating NADPH oxidases.

Research Products

• [Journal Article] Prevention of Treacher Collins syndrome craniofacial anomalies in mouse models via maternal antioxidant supplementation (2016)
  • Author(s): Daisuke Sakai, Jill Dixon, Annita Achilleos, Michael Dixon, and Paul A Trainor
  • Journal Title: Nature Communications Volume: 7 Pages: 10328-10328
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevention of the craniofacial abnormalities based on the pathogenesis of Treacher Collins syndrome (2015)
  • Author(s): Daisuke Sakai, Jun Motoyama and Paul A Trainor
  • Journal Title: Congenital Anomalies Volume: 55(4)
  • Int'l Joint Research
• [Journal Article] Gene transfer techniques in whole embryo cultured post-implantation mouse embryos. (2014)
  • Author(s): Sakai D, Trainor PA
  • Journal Title: Methods in Molecular Biology Volume: 1092 Pages: 227-234
  • DOI: 10.1007/978-1-60327-292-6_15
  • ISBN: 9781603272902, 9781603272926
  • Peer Reviewed
• [Presentation] トリーチャーコリンズ症候群モデルマウスによる顎顔面奇形発症メカニズムの解明 (2015)
  • Author(s): 酒井 大輔
  • Organizer: 第５５回 日本先天異常学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川県、横浜
  • Year and Date: 2015-07-25 – 2015-07-27
  • Invited
• [Presentation] トリーチャーコリンズ症候群モデルマウスによる顎顔面奇形発症メカニズムの解明 (2015)
  • Author(s): 酒井大輔
  • Organizer: 第５５回 日本先天異常学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-07-25
  • Invited
• [Presentation] 哺乳類胚の発生過程における外環境の役割 (2014)
  • Author(s): 酒井 大輔
  • Organizer: 第１回 発生発達基礎医学研究会
  • Place of Presentation: 東北大学、宮城県、 仙台
  • Year and Date: 2014-11-28
  • Invited
• [Presentation] Anaerobic glucose metabolism regulates neural tube formation (2014)
  • Author(s): Daisuke Sakai and Jun Motoyama
  • Organizer: 第４４回日本発生生物学会
  • Place of Presentation: WINC AICHI、愛知県、 名古屋
  • Year and Date: 2014-05-27 – 2014-05-30
• [Book] Mammalian Cell Culture. Current Protocols in Essential Laboratory Technique (2015)
  • Author(s): Dennis, R. Warner, Daisuke Sakai, and Lisa, L. Sandell
  • Total Pages: 70
  • Publisher: John Wiley & Sons