Gain-of-function mechanism of Sox3 as a sex-determining gene in Oryzias dancena

• Project/Area Number: 25840092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TAKEHANA Yusuke 基礎生物学研究所, バイオリソース研究室, 助教 (60432093)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 性決定遺伝子 / 進化

Outline of Final Research Achievements

Our previous study demonstrated that Sox3 on the Y chromosome is the sex-determining gene in Oryzias dancena. To elucidate whether Sox3 is originally involved in sex determination/differentiation in Oryzias, we generated Sox3-KO Oryzias latipes using TALEN technique, and obtained loss-of-function type mutations. Homozygous mutants survived after sexual maturation, and no phenotypic defect is observed. Homozygous XX developed as phenotypic females and YY as males. Furthermore, testis structure of homozygous XY mutant looks normal, suggesting that Sox3 is not necessary for gonadal development or testis differentiation in O. latipes. These results suggest that sex-determining function of Sox3 arose through acquisition of the novel expression patten at developing gonad in Oryzias dancena.

Research Products

• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] University of Wurzburg(ドイツ)
• [Journal Article] Origin of the boundary populations in medaka (Oryzias latipes species complex). (2016)
  • Author(s): Takehana Y, Sakai M, Narita T, Sato T, Naruse K, Sakaizumi M
  • Journal Title: Zoological Science Volume: 33 Issue: 2 Pages: 125-131
  • DOI: 10.2108/zs150144
  • NAID: 40020790412
  • Peer Reviewed
• [Journal Article] Genetic Population Structure of the Japanese Grass Lizard, <i>Takydromus tachydromoides</i> (Reptilia: Squamata), Inferred from Mitochondrial Cytochrome b Variations (2016)
  • Author(s): Takehana Y, Matsuda Y, Ikuta J, Kryukov AP, Sakaizumi M
  • Journal Title: Current Herpetology Volume: 35 Issue: 1 Pages: 22-32
  • DOI: 10.5358/hsj.35.22
  • NAID: 130005128162
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Arginine vasotocin neuronal development and its projection in the adult brain of the medaka. (2016)
  • Author(s): Kagawa N, Honda A, Zenno A, Omoto R, Imanaka S, Takehana Y, Naruse K
  • Journal Title: Neuroscience Letters Volume: 613 Pages: 47-53
  • DOI: 10.1016/j.neulet.2015.12.049
  • Peer Reviewed
• [Journal Article] Turnover of sex chromosomes in celebensis group medaka fishes. (2015)
  • Author(s): Myosho T, Takehana Y, Hamaguchi S, Sakaizumi M
  • Journal Title: G3 Volume: 5 Issue: 12 Pages: 2685-2691
  • DOI: 10.1534/g3.115.021543
  • Peer Reviewed
• [Journal Article] Genetic similarity of the Hainan medaka populations collected from hyper- and hypoosmotic environments in northern Vietnam. (2015)
  • Author(s): Hideki Hayakawa, Quang Dung Le, Masato Kinoshita, Yusuke Takehana, Kei Sakuma, Hirohiko Takeshima, Shigeaki Kojima, Kiyoshi Naruse, Koji Inoue
  • Journal Title: Ocean Science Journal Volume: 50 Issue: 2 Pages: 231-235
  • DOI: 10.1007/s12601-015-0020-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena (2014)
  • Author(s): Takehana, Y., Matsuda, M., Myosho, T., Suster, M.L., Kawakami, K., Shin-I, T., Kohara, Y., Kuroki, Y., Toyoda, A., Fujiyama, A., Hamaguchi, S., Sakaizumi, M., and Naruse, K.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4157-4157
  • DOI: 10.1038/ncomms5157
  • Peer Reviewed / Open Access
• [Journal Article] Divergence of Repetitive DNA Sequences in the Heterochromatin of Medaka Fishes: Molecular Cytogenetic Characterization of Constitutive Heterochromatin in Two Medaka species: Oryzias hubbsi and O. celebensis (Adrianichthyidae, Beloniformes) (2013)
  • Author(s): Uno Y, Asada Y, Nishida C, Takehana Y, Sakaizumi, Matsuda Y
  • Journal Title: Cytogenetic and Genome Research Volume: 141 Issue: 2-3 Pages: 212-226
  • DOI: 10.1159/000354668
  • Peer Reviewed
• [Journal Article] Sub-functionalization of duplicated genes in the evolution of nine-spined stickleback hatching enzyme (2013)
  • Author(s): Mari Kawaguchi
  • Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution Volume: 320 Issue: 3 Pages: 140-150
  • DOI: 10.1002/jez.b.22490
  • Peer Reviewed
• [Presentation] Sex-determining locus of the medaka-related fish, Oryzias haugiangensis (2017)
  • Author(s): Takehana Y, Yasugi M, Nagano A, Naruse K
  • Organizer: 8th Aquatic Animal Models of Human Disease Conference
  • Place of Presentation: The University of Alabama at Birmingham, Birmingham, USA
  • Year and Date: 2017-01-07
  • Int'l Joint Research
• [Presentation] Identification of the sex-determining locus in the medaka-related fish, Oryzias haugiangensis collected at southern Vietnam (2016)
  • Author(s): Takehana Y, Yasugi M, Nagano A, Naruse K
  • Organizer: 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: OIST (Onna-son)/Okinawa Convention Center (Naha), Okinawa
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Repeated co-option of Sox3 as the sex-determining gene in Oryzias (2016)
  • Author(s): Takehana Y, Myosho T, Hamaguchi S, Sakaizumi M, Naruse K
  • Organizer: 8th International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: The University of Hong Kong, Hong Kong, China
  • Year and Date: 2016-06-20
  • Int'l Joint Research / Invited
• [Presentation] Repeated independent evolution of Sox3 as the sex-determining gene in Oryzias. (2015)
  • Author(s): Takehana Y, Myosho T, Hamaguchi S, Sakaizumi M, Naruse K
  • Organizer: 5th International Symposium of Oryzias Fish
  • Place of Presentation: Mahasarakham, Thailand
  • Year and Date: 2015-11-12
  • Int'l Joint Research
• [Presentation] ハウザンメダカ性決定遺伝子座の同定 (2015)
  • Author(s): 竹花佑介，八杉公基，永野惇，成瀬清
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ、新潟市
  • Year and Date: 2015-09-17
• [Presentation] Co-option of Sox3 as the sex-determining factor in a medaka fish Oryzias dancena. (2015)
  • Author(s): Yusuke Takehana, Kiyoshi Naruse
  • Organizer: 7th International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Hawaii （USA）
  • Year and Date: 2015-04-13 – 2015-04-17
• [Presentation] Co-option of Sox3 as the sex-determining factor in a medaka fish Oryzias dancena. (2015)
  • Author(s): Takehana Y, Naruse K
  • Organizer: 7th International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona, Hawaii, USA
  • Year and Date: 2015-04-13
  • Int'l Joint Research
• [Presentation] インドメダカSox3YはメダカでもXX 性転換を誘導する (2014)
  • Author(s): 竹花佑介、成瀬清
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] メダカ属におけるZW型性決定様式の単系統的起源 (2013)
  • Author(s): 竹花佑介，成瀬清
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学（岡山市，岡山県）
• [Presentation] メダカ属における新奇性決定遺伝子の進化 (2013)
  • Author(s): 竹花佑介，濱口哲，酒泉満，成瀬清
  • Organizer: 日本進化学会大15回大会
  • Place of Presentation: 筑波大学（つくば市，茨城県）
• [Presentation] Evolution of the neo-Y chromosome through co-option of Sox3 in a medaka-related fish. (2013)
  • Author(s): Takehana Y, Matsuda M, Masuyama H, Myosho T, Suster ML, Kawakami K, Shin-I T, Kohara Y, Kuroki Y, Toyoda A, Fujiyama A, Hamaguchi S, Sakaizumi M, Naruse K
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: Barcelona, Spain
• [Remarks] プレスリリース「インドメダカの性決定遺伝子を発見～性染色体の多様化機構の一端を解明～」
  • URL: http://www.nibb.ac.jp/pressroom/news/2014/06/20.html