Elucidation of mechanisms that establish boundaries of dendritic fields in sensory neurons

• Project/Area Number: 25840094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo (2014); 公益財団法人大阪バイオサイエンス研究所 (2013)
• Principal Investigator: YASUNAGA KEI-ICHIRO 東京大学, 理学系研究科, 研究員 (20534572)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 受容野境界 / Wnt5 / Ryk / drl / 神経ー表皮間相互作用 / in vivo イメージング / 感覚神経 / ショウジョウバエ / Wnt / 受容野 / 樹状突起 / ライブイメージング / in vivo / RhoGEF / サイズ / 神経表皮間相互作用

Outline of Final Research Achievements

In this study, we investigated molecular and cellular mechanisms by which boundaries of dendritic fields were established in Drosophila sensory neurons. Using a combination of in vivo imaging techniques and molecular genetics, we found that Wnt5 protein prevented dendritic branches from growing across the boundary during the dendrite development.

Research Products

• [Journal Article] Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons (2015)
  • Author(s): Takahiro Kanamori, Jiro Yoshino, Kei-ichiro Yasunaga, Yusuke Dairyo, Kazuo Emoto
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6515-6515
  • DOI: 10.1038/ncomms7515
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Compartmentalized Calcium Transients Trigger Dendrite Pruning in Drosophila Sensory Neurons (2013)
  • Author(s): Takahiro Kanamori, Makoto I. Kanai, Yusuke Dairyo, Kei-ichiro Yasunaga, Rei K. Morikawa, and Kazuo Emoto
  • Journal Title: Science Volume: 340 Issue: 6139 Pages: 1475-1478
  • DOI: 10.1126/science.1234879
  • Peer Reviewed
• [Journal Article] Select interneuron clusters determine female sexual receptivity in Drosophila. (2013)
  • Author(s): Sakurai, A., Koganezawa, M., Yasunaga, K., Emoto, K. and Yamamoto, D.
  • Journal Title: Nat. Commun. Volume: 4 Issue: 1 Pages: 1825-1833
  • DOI: 10.1038/ncomms2837
  • Peer Reviewed
• [Presentation] Adult Drosophila sensory neurons use two distinct mechanisms to specify their dendritic fields (2013)
  • Author(s): Kei-ichiro Yasunaga, Kazuo Emoto
  • Organizer: Neuro2013
  • Place of Presentation: Kyoto
• [Remarks] 東京大学大学院理学系研究科生物科学専攻脳機能学分野ホームページ
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/brain/
• [Remarks] 東京大学大学院理学系研究科生物科学専攻脳機能学講座ホームページ
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/brain/index.html