Study on protein complexes of NAC transcription factor family

• Project/Area Number: 25840098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: YAMAGUCHI Masatoshi 埼玉大学, 理工学研究科, 准教授 (20373376)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: NACドメイン転写因子 / タンパク質相互作用 / 道管分化

Outline of Final Research Achievements

NAC transcription factor family has been known to be involved in various biological regulations during plant development. Previously, we isolated NAC transcription factors, VNI1 and VNI2, as interacting factors with another NAC transcription factor VND7, a key regulator of xylem vessel formation. Here, we demonstrated that NAC domain transcription factors VNI1 and its closest homolog, ANAC103, are able to form protein complexes with several NAC domain transcription factors. In addition, by using a cDNA library for yeast two-hybrid screening containing full-length transcription factors, we succeeded in isolation of 30 members of NAC transcription factors as interacting factors with VNI2. Some of them are known to be involved in stress responses or senescence, suggesting that protein complexes of NAC transcription factor may play pivotal roles in various biological events as well as xylem vessel formation.

Research Products

• [Journal Article] Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays. (2015)
  • Author(s): Kitano S, Miyagi A, Oono Y, Hase Y, Narumi I, Yamaguchi M, Uchimiya H, Kawai-Yamada M
  • Journal Title: Metabolomics Volume: 11 Issue: 1 Pages: 134-142
  • DOI: 10.1007/s11306-014-0684-4
  • Peer Reviewed / Open Access
• [Journal Article] Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation (2015)
  • Author(s): Endo H, Yamaguchi M, Tamura T, Nakano Y, Nishikubo N, Yoneda A, Kato K, Kubo M, Kajita S, Katayama Y, Ohtani M, Demura T
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 2 Pages: 242-254
  • DOI: 10.1093/pcp/pcu134
  • Peer Reviewed / Open Access
• [Journal Article] Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins (2015)
  • Author(s): Yamaguchi M, Nagahage ISP, Ohtani M, Ishikawa T, Uchimiya H, Kawai-Yamada M, Demura T
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 2 Pages: 119-123
  • DOI: 10.5511/plantbiotechnology.15.0208a
  • NAID: 130005085996
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of NAC transcription factors to plant adaptation to land. (2014)
  • Author(s): Xu, B., Ohtani, M., Yamaguchi, M., Toyooka, K., Wakazaki, M., Sato, M., Kubo, M., Nakano, Y., Sano, R., Hiwatashi, Y., Murata, T., Kurata, T., Yoneda, A., Kato, K., Hasebe, M., and Demura, T.
  • Journal Title: Science Volume: 343 Issue: 6178 Pages: 1505-1508
  • DOI: 10.1126/science.1248417
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. (2014)
  • Author(s): Kakimoto M, ahikawa T, Miyagi A, Saito K, Miyazaki M, Asaeda T, Yamaguchi M, Uchimiya H, and Kawai-Yamada M.
  • Journal Title: J. Plant Physiol. Volume: 171 Issue: 3-4 Pages: 292-300
  • DOI: 10.1016/j.jplph.2013.09.005
  • Peer Reviewed
• [Journal Article] <i>ATL54</i>, a ubiquitin ligase gene related to secondary cell wall formation, is transcriptionally regulated by MYB46 (2013)
  • Author(s): Noda,S.,et al.
  • Journal Title: Plant Biotechnology Volume: 30 Issue: 5 Pages: 503-509
  • DOI: 10.5511/plantbiotechnology.13.0905b
  • NAID: 130003382501
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] ATL54, a RING-H2 domain protein selected by a gene co-expression network analysis, is associated with secondary cell wall formation in <i>Arabidopsis</i> (2013)
  • Author(s): Noda, S.,et al.
  • Journal Title: Plant Biotechnology Volume: 30 Issue: 2 Pages: 169-177
  • DOI: 10.5511/plantbiotechnology.13.0304a
  • NAID: 10031184121
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] A computational and experimental approach reveals that the 5′-proximal region of the 5′-UTR has a Cis-regulatory signature responsible for heat stress-regulated mRNA translation in Arabidopsis. (2013)
  • Author(s): Matsuura,H.,et al.
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 4 Pages: 474-483
  • DOI: 10.1093/pcp/pcs189
  • Peer Reviewed
• [Presentation] RING finger proteins regulate protein stability of VNI2. (2015)
  • Author(s): Yamaguchi M
  • Organizer: Front Lines of Plant Cell Wall Research
  • Place of Presentation: Todaiji temple (Nara, Nara, Japan)
  • Year and Date: 2015-03-20 – 2015-03-21
• [Presentation] 道管形成を負に制御する転写抑制因子VNI2のタンパク質制御機構 (2015)
  • Author(s): 北川純子、松田浩平、加藤晃、川合真紀、出村拓、山口雅利
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都、世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] BRITTLE CULM 4 mutant decreases in secondary cell wall formation in rice. (2014)
  • Author(s): Yamaguchi M, Sato A, Yoshimura M, Kurata T, Kawai-Yamada M, Kawasaki S, Tsumuraya Y, Kotake T
  • Organizer: 5th International Conference Plant Cell Wall Biology 27-31- July 2014
  • Place of Presentation: Hotel Grand Chancellor Palm Cove (Palm Cove, Queensland, Australia)
  • Year and Date: 2014-07-27 – 2014-07-31
• [Presentation] VND-INTERAACTING PROTEIN2 is controlled by ubiquitin-mediated proteolysis. (2013)
  • Author(s): Yamaguchi M, Matsuda K, Kato K, Demura T
  • Organizer: 13th Cell Wall Meeting
  • Place of Presentation: Nantes, France
• [Presentation] Transcriptional regulation of secondary cell wall synthesis. (2013)
  • Author(s): Yamaguchi M
  • Organizer: UK-Japan Joint Meeting on Plant Cell Biology
  • Place of Presentation: Cambridge, UK
  • Invited
• [Presentation] 道管分化を制御する遺伝子転写ネットワークの解析 (2013)
  • Author(s): 山口 雅利
  • Organizer: 日本植物細胞分生物学会
  • Place of Presentation: 北海道大学（北海道）
  • Invited
• [Remarks] TOP of 山口研究グループ
  • URL: http://park.saitama-u.ac.jp/~myamaguchi/index.html