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Analysis of the transcription factors that control nitrate responses in higher plants

Research Project

Project/Area Number 25840099
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

KONISHI Mineko  東京大学, 生物生産工学研究センター, 特任助教 (20642341)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords植物栄養 / 硝酸シグナル / 硝酸シグナル応答
Outline of Final Research Achievements

I analyzed molecular characteristics of eight NLP proteins out of nine NLP transcription factors in Arabidopsis, which are presumably involved in nitrate-responsive gene expression. Although all NLP proteins analyzed were activated in response to nitrate signal, NLP6 and NLP7 were more strongly activated by nitrate, compared to others. Furthermore, by generating various multiple nlp mutants, NLP6 and NLP7 were found to contribute to biomass of shoots in Arabidopsis, while contribution of other NLP proteins to biomass was not apparent.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (7 results)

  • [Journal Article] MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells2015

    • Author(s)
      Konishi, M., Donner, T. J., Scarpella, E. and Yanagisawa, S.
    • Journal Title

      Journal of Experimental Botany

      Volume: 66 Issue: 1 Pages: 283-291

    • DOI

      10.1093/jxb/eru418

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptional repression caused by Dof5.8 is involved in proper vein network formation in Arabidopsis thaliana leaves2015

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      Journal of Plant Research

      Volume: 印刷中 Issue: 4 Pages: 643-652

    • DOI

      10.1007/s10265-015-0712-0

    • NAID

      40020510808

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nitrite transport activity of a novel HPP family protein conserved in cyanobacteria and chloroplasts2014

    • Author(s)
      Maeda, S., Konishi, M., Yanagisawa, S. and Omata, T.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 7 Pages: 1311-1324

    • DOI

      10.1093/pcp/pcu075

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Emergence of a new step towards understanding the molecular mechanisms underlying nitrate-regulated gene expression2014

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      Journal of Experimental Botany

      Volume: 65 Issue: 19 Pages: 5589-5600

    • DOI

      10.1093/jxb/eru267

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 植物の硝酸シグナル応答機構-NIN様タンパク質が硝酸シグナル応答を司る2014

    • Author(s)
      小西美稲子, 柳澤修一
    • Journal Title

      化学と生物

      Volume: 52 Pages: 421-423

    • NAID

      130005084893

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 胚発生と維管束パターン形成におけるシロイヌナズナDof5.8転写因子の役割2015

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本植物生理学会2015年度年会
    • Place of Presentation
      東京農業大学世田谷キャンパス (東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 硝酸シグナル応答を制御するNIN/NLPファミリータンパク質の転写促進活性と進化的考察2014

    • Author(s)
      小西美稲子、鈴木渉、柳澤修一
    • Organizer
      日本植物学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 硝酸シグナル応答性転写因子による地上部と根の生長の制御2014

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会 2014年度大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物の硝酸応答機構研究の新展開2014

    • Author(s)
      柳澤修一、小西美稲子、鈴木渉
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学(富山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 高等植物の硝酸応答を担うNLP転写因子の機能ドメインの解析2014

    • Author(s)
      小西美稲子、鈴木渉、柳澤修一
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学(富山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Nitrate-reponsive transcription factor NLP directly regulates expression of BT protein genes2014

    • Author(s)
      Nozomi Yoshioka, Mineko Konishi, Tetsuya Ishida, Shuichi Yanagisawa
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学(富山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] NIN-like proteinによる硝酸誘導性遺伝子発現の制御2013

    • Author(s)
      小西美稲子、鈴木渉、柳澤修一
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学(愛知県)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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