Analysis of the physiological roles and dynamics of TGN in plant cell

• Project/Area Number: 25840100
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Uemura Tomohiro 東京大学, 理学(系)研究科(研究院), 助教 (90415092)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: オルガネラ / ＴＧＮ / ライブイメージング / 病原菌応答 / TGN / 塩ストレス / シロイヌナズナ / SNARE

Outline of Final Research Achievements

The trans-Golgi network (TGN) functions as a sorting station that directs cargo proteins to a variety of destinations including post-Golgi compartments and the extracellular space. However, the functions and dynamics of the TGN in plant cells have remained unknown. To reveal the dynamics of the plant TGN, transgenic plants expressing GFP-SYP43 as a TGN marker was observed. Observations revealed that there are two types of TGN in Arabidopsis root: the GA-TGNs (Golgi-associated TGNs), located on the trans-side of the Golgi apparatus, and the GI-TGNs (Golgi-released independent TGNs), located away from the Golgi apparatus and behaving independently. The GI-TGNs is segregated from the GA-TGNs. I also found that the number of the GI-TGNs are different among observed tissues.

Research Products

• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Journal Article] SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery. (2016)
  • Author(s): Kanazawa, T., Era, A., Minamino, N., Shikano, Y., Fujimoto, M., Uemura, T., Nishihama, R., Yamato, KT., Ishizaki, K., Nishiyama, T., Kohchi, T., Nakano, A., Ueda, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 2 Pages: 307-24
  • DOI: 10.1093/pcp/pcv076
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of RAB5-positive multivesicular endosomes and the trans-Golgi network in root meristematic cells of Arabidopsis thaliana. (2016)
  • Author(s): Ito, E., Uemura, T. Ueda, T. and Nakano, A.
  • Journal Title: Plant Biotechnology Volume: 印刷中
  • NAID: 130005281074
  • Peer Reviewed
• [Journal Article] Dynamic behavior of the trans-Golgi network in root tissues of Arabidopsis revealed by super-resolution live imaging (2014)
  • Author(s): T. Uemura, Y. Suda, T. Ueda, and A. Nakano
  • Journal Title: Plant Cell Physiol Volume: 55 Issue: 4 Pages: 694-670
  • DOI: 10.1093/pcp/pcu010
  • Peer Reviewed
• [Journal Article] A unique HEAT repeat-containing protein SHOOT GRAVITROPISM 6 is involved in vacuolar membrane dynamics in gravity sensing cells of Arabidopsis inflorescence stem (2014)
  • Author(s): Hashiguchi, Y., Yano, D., Nagafusa, K., Kato, T., Saito, C., Uemura, T., Ueda, T., Nakano, A., Tasaka, M. and Morita, MT
  • Journal Title: Plant Cell Phys. Volume: 55 Issue: 4 Pages: 811-822
  • DOI: 10.1093/pcp/pcu020
  • Peer Reviewed
• [Journal Article] The ESCRT-III interacting deubiquitinating enzyme AMSH3 is essential for degradation of ubiquitinated membrane proteins in Arabidopsis thaliana. (2014)
  • Author(s): Katsiarimpa, A., Muñoz, A., Kalinowska, K., Uemura, T., Rojo, E. and Isono, E.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 4 Pages: 727-736
  • DOI: 10.1093/pcp/pcu019
  • Peer Reviewed
• [Journal Article] Interactomics of Qa-SNARE in Arabidopsis thaliana (2014)
  • Author(s): Fujiwara, M., Uemura, T., Ebine, K., Nishimori, Y., Ueda, T., Nakano, A., Sato, MH. and Fukao, Y.
  • Journal Title: Plant Cell Phys. Volume: 55 Issue: 4 Pages: 781-789
  • DOI: 10.1093/pcp/pcu038
  • Peer Reviewed / Open Access
• [Journal Article] Sex-specific post-translational regulation of the gamete fusogen GCSI in the isogamous volvocine alga Gonium pectorale. (2014)
  • Author(s): Kawai-Toyooka H, Mori T, Hamaji T, Suzuki M, Olson BJ, Uemura T, Ueda T, Nakano A, Toyoda A, Fujiyama A, Nozaki H
  • Journal Title: EUkaryotic Cell Volume: (in press) Issue: 5 Pages: 648-656
  • DOI: 10.1128/ec.00330-13
  • Peer Reviewed
• [Journal Article] Plant vacuolar trafficking occurs through distinctly regulated pathways. (2014)
  • Author(s): Ebine K, Inoue T, Ito J, Ito E, Uemura T, Goh T, Abe H, Sato K, Nakano A, Ueda T.
  • Journal Title: Curr Biol. Volume: 24 Issue: 12 Pages: 1375-1382
  • DOI: 10.1016/j.cub.2014.05.004
  • Peer Reviewed
• [Journal Article] The powdery mildew resistance protein RPW8.2 is carried on VAMP721/722 vesicles to the extrahaustorial membrane of haustorial complexes (2014)
  • Author(s): 7.Kim, H., O'Connell, R., Maekawa-Yoshikawa, M., Uemura, T., Neumann, U., Schulze-Lefert P
  • Journal Title: Plant J. Volume: 79 Issue: 5 Pages: 835-847
  • DOI: 10.1111/tpj.12591
  • Peer Reviewed
• [Journal Article] Formation and Maintenance of the Golgi Apparatus in Plant Cells (2014)
  • Author(s): Yoko Ito, Tomohiro Uemura, and Akihiko Nakano
  • Journal Title: International Review of Cell & Molecular Biology Volume: 310 Pages: 221-287
  • DOI: 10.1016/b978-0-12-800180-6.00006-2
  • ISBN: 9780128001806
  • Peer Reviewed
• [Journal Article] Plant vacuolar trafficking driven by RAB and SNARE proteins (2014)
  • Author(s): Uemura, T. and Ueda, T.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 22 Pages: 116-121
  • DOI: 10.1016/j.pbi.2014.10.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance. (2013)
  • Author(s): Rin Asaoka, Tomohiro Uemura, Jun Ito, Masaru Fujimoto, Emi Ito, Takashi Ueda, Akihiko Nakano
  • Journal Title: The Plant Journal Volume: 73 Issue: 2 Pages: 240-249
  • DOI: 10.1111/tpj.12023
  • Peer Reviewed
• [Journal Article] RABA Members Act in Distinct Steps of Subcellular Trafficking of the FLAGELLIN SENSING2 Receptor. (2013)
  • Author(s): Choi, et al.
  • Journal Title: Plant Cell Volume: (in press) Issue: 3 Pages: 1174-1187
  • DOI: 10.1105/tpc.112.108803
  • Peer Reviewed
• [Journal Article] Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis thaliana. (2013)
  • Author(s): Tanaka H, Kitakura S, Rakusová H , Uemura T, Feraru MI, De Rycke R, Robert S, Kakimoto T, Friml J.
  • Journal Title: PLoS Genet. Volume: 9 Issue: 5 Pages: e1003540-e1003540
  • DOI: 10.1371/journal.pgen.1003540
  • Peer Reviewed
• [Journal Article] New insights into the role of Arabidopsis RABA1 GTPases in salinity stress tolerance. (2013)
  • Author(s): Asaoka Rin, Uemura Tomohiro, Nishida Sho, Fujiwara Toru, Ueda Takashi, Nakano Akihiko
  • Journal Title: Plant Signal and Behavior Volume: 8 Issue: 9 Pages: e25377-e25377
  • DOI: 10.4161/psb.25377
  • Peer Reviewed / Open Access
• [Journal Article] Plant TGNs: dynamics and physiological functions (2013)
  • Author(s): Tomohiro Uemura and Akihiko Nakano
  • Journal Title: Histochem. Cell Biol. Volume: 140 Issue: 3 Pages: 341-345
  • DOI: 10.1007/s00418-013-1116-7
  • Peer Reviewed
• [Journal Article] Physical interaction of noral organs contr01S petal morphogenesiS in Arabidopsis thaliana. (2013)
  • Author(s): Seiji Takeda, et al.
  • Journal Title: Plant Physiol. Volume: 61 Issue: 3 Pages: 1242-1250
  • DOI: 10.1104/pp.112.212084
  • Peer Reviewed
• [Presentation] The dynamics and physiological roles of the TGN in plants. (2016)
  • Author(s): Tomohiro Uemura
  • Organizer: Progress100: Second international symposium: Protein Trafficking and Intracellular Signaling of Plant and Fungal Cells
  • Place of Presentation: Hakata, Japan
  • Year and Date: 2016-02-08
  • Int'l Joint Research / Invited
• [Presentation] 植物におけるトランスゴルジネットワーク（ＴＧＮ）の動態と生理機能 (2015)
  • Author(s): 植村知博
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（神戸・兵庫）
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants. (2015)
  • Author(s): Tomohiro Uemura, Takashi Ueda, and Akihiko Nakano
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] 植物におけるTGNの動態と生理機能 (2015)
  • Author(s): 植村知博、中野明彦
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール堀船（江戸川区・東京）
  • Year and Date: 2015-06-30
• [Presentation] ポストゴルジオルガネラが担う環境ストレス応答 (2015)
  • Author(s): 植村知博
  • Organizer: 第17回植物オルガネラワークショップ
  • Place of Presentation: 東京大学、東京都目黒区
  • Year and Date: 2015-03-15
  • Invited
• [Presentation] 植物におけるトランスゴルジネットワーク（ＴＧＮ）の動態 (2014)
  • Author(s): 植村知博、中野明彦
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2014-11-27
  • Invited
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants (2014)
  • Author(s): Tomohiro Uemura and Akihiko Nakano
  • Organizer: 38th Naito Conference on Molecule-based biological systems
  • Place of Presentation: SAPPORO, Japan
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] 植物におけるポストゴルジオルガネラの動態と生理機能の研究 (2014)
  • Author(s): 植村知博
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学、川崎市
  • Year and Date: 2014-09-13
  • Invited
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants. (2014)
  • Author(s): Tomohiro Uemura, Takashi Ueda, Akihiko Nakano
  • Organizer: 17th European Network for plant Endomembrane Research Meeting
  • Place of Presentation: Lecce, Italy
  • Year and Date: 2014-09-08 – 2014-09-11
• [Presentation] The dynamics and functions of Qa-SNAREs localized on the trans-Golgi network on response to Colletotrichum gloeosporioides. (2014)
  • Author(s): Tomohiro Uemura, Takashi Shimada, Takashi Ueda, Akihiko Nakano
  • Organizer: IS-MPMI 2014 XVI International Congress
  • Place of Presentation: Rhodes, Greece
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants. (2014)
  • Author(s): Tomohiro Uemura, Yasuyuki Suda, Takashi Ueda and Akihiko Nakano.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂、奈良市
  • Year and Date: 2014-06-12
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants. (2013)
  • Author(s): Tomohiro Uemura
  • Organizer: Golgi Apparatus Symposium
  • Place of Presentation: Bad Ischl, Austria
  • Invited