Multiple approach on Study on the mechanism of polar localization of auxin efflux carrier PIN protein
| Project/Area Number |
25840101
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tohoku University (2015)
The University of Tokyo (2013-2014) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | オーキシン / 細胞極性 / 小胞輸送 / 極性輸送 / PIN / VAN3 / GNOM / 極性形成 / 細胞膜ドメイン / 細胞壁 |
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| Outline of Final Research Achievements |
To elucidate the molecular mechanism of cell polarity establishment in plants, I analyzed the detailed distribution of PIN proteins at plasma membranes (PM)s. I identified that PIN proteins formed dot-like clusters at PMs and they are stable cellular compartments that do not diffuse across PMs. I also identified that PIN clusters are established or maintained by the cell wall components as well as the unknown function protein MAB4. Furthermore, I analyzed the van2, van5 and van6 mutants that are defective in vein development，related to polar auxin transport. Based on whole genome sequence analysis, I identified several candidates of responsible genes for van2, van5 and van6 mutants. Within them, I identified that responsible gene of van5 mutants is involved in sterol biosynthesis, which further confirms that appropriate lipid compositions of membranes are critical to establish cell polarity.
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Report
(4 results)
Research Products
(8 results)
