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Functional analysis of SNS1, a novel negative regulator of ABA signaling.

Research Project

Project/Area Number 25840103
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

UMEZAWA Taishi  東京農工大学, (連合)農学研究科(研究院), 准教授 (70342756)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsアブシシン酸 / シロイヌナズナ / リン酸化 / SnRK2 / プロテオーム
Outline of Final Research Achievements

Abscisic acid (ABA) is a phytohormone which is important for stress responses in plants. Recent studies demonstrated that the ABA signaling pathway consists of three core components, receptor, protein phosphatase and protein kinase (SnRK2), and SnRK2 phosphorylates its substrates to transmit ABA signals. Our previous study have identified SNS1 as a SnRK2 substrate, however, its function was unknown. Then we performed a reverse genetic study, and demonstrated that SNS1 is a negative regulator of ABA signaling either in seeds or vegetative tissues. Furthermore, a trascriptome analysis using RNA-seq revealed that SNS1 regulates a part of ABA-responsive genes, as well as flowering-related genes.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Invited: 5 results) Book (1 results)

  • [Journal Article] Screening of kinase substrates using kinase-knockout mutants.2015

    • Author(s)
      Umezawa, T.
    • Journal Title

      Methods in Molecular Biology

      Volume: **

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation Networks in the Abscisic Acid Signaling Pathway.2014

    • Author(s)
      Umezawa, T., Takahashi, F. and Shinozaki, K.
    • Journal Title

      The Enzymes

      Volume: 35 Pages: 27-56

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetics and Phosphoproteomics Reveal a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis thaliana.2013

    • Author(s)
      Umezawa T, Sugiyama N, Takahashi F, Anderson JC, Ishihama Y, Peck SC, Shinozaki K.
    • Journal Title

      Sci Signal.

      Volume: 6 Issue: 270

    • DOI

      10.1126/scisignal.2003509

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナ種子におけるアブシジン酸シグナルネットワークのリン酸化プロテオーム解析2015

    • Author(s)
      天谷杏奈,中神弘史,平山隆志,梅澤泰史
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナのアブシジン酸応答に関わる機能未知タンパク質SNS1の解析2014

    • Author(s)
      鈴木梨沙、石塚梢、梅澤泰史
    • Organizer
      第78回日本植物学会年会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] ABAシグナル伝達系におけるCグループMAPキナーゼの機能解析2014

    • Author(s)
      廣谷美咲,高橋史憲,篠崎一雄,梅澤泰史
    • Organizer
      第78回日本植物学会年会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメツリガネゴケにおけるABAシグナル伝達経路のリン酸化プロテオーム解析2014

    • Author(s)
      本多慶匡,杉山直幸,桑村真由里,寺尾亮佑,石塚梢,坂田洋一,竹澤大輔,篠崎一雄,梅澤泰史
    • Organizer
      第78回日本植物学会年会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] PHOSPHOPROTEOMIC APPROACHES TOWARD UNDERSTANDING THE ABA SIGNALING NETWORK IN LAND PLANTS2014

    • Author(s)
      Yoshimasa Honda, Naoyuki Sugiyama, Mayuri Kuwamura, Ryosuke Terao, Kozue Ishizuka, Yoichi Sakata, Daisuke Takezawa, Kazuo Shinozaki, Yasushi Ishihama, Taishi Umezawa
    • Organizer
      The 6th International Symposium on Frontiers in Agricultural Proteome Research, the 1st AOAPO conference, & the 5th Plant Proteomics Conference in China
    • Place of Presentation
      ハルビン(中国)
    • Year and Date
      2014-06-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナABA応答性転写因子AREB1のSer45はSnRK2のリン酸化標的部位である2014

    • Author(s)
      寺尾亮佑・篠崎一雄・梅澤泰史
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] SnRK2-substrate 1はABAシグナル伝達を負に制御する2014

    • Author(s)
      石塚梢・鈴木梨沙・古崎俊則・石井一夫・篠崎一雄・梅澤泰史
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒメツリガネゴケにおけるABAシグナル伝達経路のリン酸化プロテオーム解析2014

    • Author(s)
      本多慶匡・杉山直幸・桑村真由里・寺尾亮佑・石塚梢・坂田洋一・竹澤大輔・篠崎一雄・石濱泰・梅澤泰史
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphoproteomic Approaches Toward Understanding the ABA Signaling Network in Land Plants2013

    • Author(s)
      Umezawa, T.
    • Organizer
      Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms - from cyanobacteria to land plants"
    • Place of Presentation
      ハイデラバード大学(インド)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 遺伝学とリン酸化プロテオミクスの融合によるアブシシン酸シグナル伝達系の包括的な解析2013

    • Author(s)
      梅澤泰史・杉山直幸・高橋史憲・Jeffery Anderson・坂田洋一・竹澤大輔・石濱泰・Scott Peck・篠崎一雄
    • Organizer
      第86日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Integrating Genetics and Phosphoproteomics Reveals a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis.2013

    • Author(s)
      Umezawa, T., Sugiyama, N., Takahashi, F., Anderson, J.C., Ishihama, Y., Peck, S.C. and Shinozaki, K.
    • Organizer
      HUPO 12th Annual World Congress
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphoproteomic Approaches to Understanding ABA Signaling Networks in Plants.2013

    • Author(s)
      Umezawa, T.
    • Organizer
      Gordon Research Conference - ‘Posttranslational Modification Networks: Phosphosignaling”
    • Place of Presentation
      香港科技大学(香港)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Integrating Genetics and Phophoproteomics Reveals a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis.2013

    • Author(s)
      Umezawa, T., Sugiyama, N., Takahashi, F., Anderson, J. C., Terao, R., Ishizuka, K., Ishihama, Y., Peck, S. C. and Shinozaki, K.
    • Organizer
      24th International Conference of Arabidopsis Research 2013
    • Place of Presentation
      シドニーコンベンションセンター(オーストラリア)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] “Protein Phosphorylation Network” in Plant and Microbe Adaptations to Cold in a Changing World2013

    • Author(s)
      Umezawa, T., Sugiyama, N., Anderson, J. C., Takahashi, F., Ishihama, Y., Peck, S. C. and Shinozaki, K.
    • Total Pages
      352
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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