Functional analysis of SNS1, a novel negative regulator of ABA signaling.

• Project/Area Number: 25840103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: UMEZAWA Taishi 東京農工大学, (連合)農学研究科(研究院), 准教授 (70342756)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アブシシン酸 / シロイヌナズナ / リン酸化 / SnRK2 / プロテオーム

Outline of Final Research Achievements

Abscisic acid (ABA) is a phytohormone which is important for stress responses in plants. Recent studies demonstrated that the ABA signaling pathway consists of three core components, receptor, protein phosphatase and protein kinase (SnRK2), and SnRK2 phosphorylates its substrates to transmit ABA signals. Our previous study have identified SNS1 as a SnRK2 substrate, however, its function was unknown. Then we performed a reverse genetic study, and demonstrated that SNS1 is a negative regulator of ABA signaling either in seeds or vegetative tissues. Furthermore, a trascriptome analysis using RNA-seq revealed that SNS1 regulates a part of ABA-responsive genes, as well as flowering-related genes.

Research Products

• [Journal Article] Screening of kinase substrates using kinase-knockout mutants. (2015)
  • Author(s): Umezawa, T.
  • Journal Title: Methods in Molecular Biology Volume: **
  • Peer Reviewed
• [Journal Article] Phosphorylation Networks in the Abscisic Acid Signaling Pathway. (2014)
  • Author(s): Umezawa, T., Takahashi, F. and Shinozaki, K.
  • Journal Title: The Enzymes Volume: 35 Pages: 27-56
  • Peer Reviewed
• [Journal Article] Genetics and Phosphoproteomics Reveal a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis thaliana. (2013)
  • Author(s): Umezawa T, Sugiyama N, Takahashi F, Anderson JC, Ishihama Y, Peck SC, Shinozaki K.
  • Journal Title: Sci Signal. Volume: 6 Issue: 270
  • DOI: 10.1126/scisignal.2003509
  • Peer Reviewed
• [Presentation] シロイヌナズナ種子におけるアブシジン酸シグナルネットワークのリン酸化プロテオーム解析 (2015)
  • Author(s): 天谷杏奈，中神弘史，平山隆志，梅澤泰史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-15 – 2015-03-17
• [Presentation] シロイヌナズナのアブシジン酸応答に関わる機能未知タンパク質SNS1の解析 (2014)
  • Author(s): 鈴木梨沙、石塚梢、梅澤泰史
  • Organizer: 第78回日本植物学会年会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ABAシグナル伝達系におけるCグループMAPキナーゼの機能解析 (2014)
  • Author(s): 廣谷美咲，高橋史憲，篠崎一雄，梅澤泰史
  • Organizer: 第78回日本植物学会年会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ヒメツリガネゴケにおけるABAシグナル伝達経路のリン酸化プロテオーム解析 (2014)
  • Author(s): 本多慶匡，杉山直幸，桑村真由里，寺尾亮佑，石塚梢，坂田洋一，竹澤大輔，篠崎一雄，梅澤泰史
  • Organizer: 第78回日本植物学会年会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] PHOSPHOPROTEOMIC APPROACHES TOWARD UNDERSTANDING THE ABA SIGNALING NETWORK IN LAND PLANTS (2014)
  • Author(s): Yoshimasa Honda, Naoyuki Sugiyama, Mayuri Kuwamura, Ryosuke Terao, Kozue Ishizuka, Yoichi Sakata, Daisuke Takezawa, Kazuo Shinozaki, Yasushi Ishihama, Taishi Umezawa
  • Organizer: The 6th International Symposium on Frontiers in Agricultural Proteome Research, the 1st AOAPO conference, & the 5th Plant Proteomics Conference in China
  • Place of Presentation: ハルビン（中国）
  • Year and Date: 2014-06-25
  • Invited
• [Presentation] シロイヌナズナABA応答性転写因子AREB1のSer45はSnRK2のリン酸化標的部位である (2014)
  • Author(s): 寺尾亮佑・篠崎一雄・梅澤泰史
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] SnRK2-substrate 1はABAシグナル伝達を負に制御する (2014)
  • Author(s): 石塚梢・鈴木梨沙・古崎俊則・石井一夫・篠崎一雄・梅澤泰史
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] ヒメツリガネゴケにおけるABAシグナル伝達経路のリン酸化プロテオーム解析 (2014)
  • Author(s): 本多慶匡・杉山直幸・桑村真由里・寺尾亮佑・石塚梢・坂田洋一・竹澤大輔・篠崎一雄・石濱泰・梅澤泰史
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] Phosphoproteomic Approaches Toward Understanding the ABA Signaling Network in Land Plants (2013)
  • Author(s): Umezawa, T.
  • Organizer: Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms - from cyanobacteria to land plants"
  • Place of Presentation: ハイデラバード大学（インド）
  • Invited
• [Presentation] 遺伝学とリン酸化プロテオミクスの融合によるアブシシン酸シグナル伝達系の包括的な解析 (2013)
  • Author(s): 梅澤泰史・杉山直幸・高橋史憲・Jeffery Anderson・坂田洋一・竹澤大輔・石濱泰・Scott Peck・篠崎一雄
  • Organizer: 第86日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] Integrating Genetics and Phosphoproteomics Reveals a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis. (2013)
  • Author(s): Umezawa, T., Sugiyama, N., Takahashi, F., Anderson, J.C., Ishihama, Y., Peck, S.C. and Shinozaki, K.
  • Organizer: HUPO 12th Annual World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] Phosphoproteomic Approaches to Understanding ABA Signaling Networks in Plants. (2013)
  • Author(s): Umezawa, T.
  • Organizer: Gordon Research Conference - ‘Posttranslational Modification Networks: Phosphosignaling”
  • Place of Presentation: 香港科技大学（香港）
  • Invited
• [Presentation] Integrating Genetics and Phophoproteomics Reveals a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis. (2013)
  • Author(s): Umezawa, T., Sugiyama, N., Takahashi, F., Anderson, J. C., Terao, R., Ishizuka, K., Ishihama, Y., Peck, S. C. and Shinozaki, K.
  • Organizer: 24th International Conference of Arabidopsis Research 2013
  • Place of Presentation: シドニーコンベンションセンター（オーストラリア）
  • Invited
• [Book] “Protein Phosphorylation Network” in Plant and Microbe Adaptations to Cold in a Changing World (2013)
  • Author(s): Umezawa, T., Sugiyama, N., Anderson, J. C., Takahashi, F., Ishihama, Y., Peck, S. C. and Shinozaki, K.
  • Total Pages: 352
  • Publisher: Springer