The analysis of circadian clock and photoperiodic flowering pathway in the leaf vascular tissue

• Project/Area Number: 25840104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University (2014-2015); Nagoya University (2013)
• Principal Investigator: Ito Shogo 京都大学, 理学(系)研究科(研究院), 助教 (60632586)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 光周性 / 花成 / 概日時計 / 組織特異性

Outline of Final Research Achievements

The circadian clock controlled photoperiodic flowering signal transduction pathways (so called CO-FT pathway) sense seasonal changes in day length and induce flowering at the most appropriate timing in Arabidopsis. Both CO and FT are mainly expressed in the companion cells of the young leaf phloem. Here, we first purified the nuclei and RNA from the specific cells in which CO and/or FT genes are expressed. Then purified tissue specific RNA was used for transcriptome analysis to obtain additional CO or FT transcriptional regulators in Arabidopsis.

Research Products

• [Int'l Joint Research] Washington University(米国)
• [Journal Article] Cool night-time temperatures induce the expression of CONSTANS and FLOWERING LOCUS T to regulate flowering in Arabidopsis (2016)
  • Author(s): Hannah A. Kinmonth-Schultz, Xinran Tong, Jae Lee, Young Hun Song, Shogo Ito, Soo-Hyung Kim, Takato Imaizumi
  • Journal Title: New Phytologist Volume: - Issue: 1 Pages: 1-17
  • DOI: 10.1111/nph.13883
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clock-Controlled and FLOWERING LOCUS T (FT)-Dependent Photoperiodic Pathway in <i>Lotus japonicus</i> I: Verification of the Flowering-Associated Function of an FT Homolog (2013)
  • Author(s): Yamashino T, Yamawaki S, Hagui E, Ueoka-Nakanishi H, Nakamichi N, Ito S, Mizuno T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 4 Pages: 747-753
  • DOI: 10.1271/bbb.120871
  • NAID: 10031170713
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Flowering time regulation: photoperiod- and temperature-sensing in leaves (2013)
  • Author(s): Song YH, Ito S, Imaizumi T
  • Journal Title: Trends Plant Sci. Volume: 18 Pages: 575-583
  • Peer Reviewed
• [Journal Article] 光周期による花成ホルモンFTの発現メカニズム (2013)
  • Author(s): 伊藤照悟
  • Journal Title: 化学と生物 Volume: 51 Pages: 782-784
  • Peer Reviewed
• [Presentation] 単子葉植物のコウキクサにおけるCaMV35S ならびにZmUBQ プロモーターの転写活性のLUCIFERASE レポーターを用いた 時空間解析 (2016)
  • Author(s): 伊藤照悟，内海陽子，小山時隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] ウキクサ植物の発光レポーター安定形質転換体を用いた概日リズム解析 (2015)
  • Author(s): 伊藤照悟，上野賢也，内海陽子，小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2015-11-21
• [Presentation] Improvement of fast and efficient Agrobacterium mediated stable transformation methods for Lemna species (2015)
  • Author(s): Shogo Ito
  • Organizer: The Third International Conference on Duckweed Research and Applications
  • Place of Presentation: Kyoto
  • Year and Date: 2015-07-03
  • Int'l Joint Research
• [Presentation] Transient and Agro-mediated stable transformation method for circadian clock analysis in Lemna species (2015)
  • Author(s): Shogo Ito
  • Organizer: RNA and Clock 2015 "Epigenetic Landscape in Biological Rhythms"
  • Place of Presentation: Awaji Yumebutai, Awaji
  • Year and Date: 2015-03-25 – 2015-03-26
• [Presentation] ウキクサ類におけるカルス誘導とアグロバクテリウム共培養による安定形質転換体作製の試み (2015)
  • Author(s): 伊藤照悟，内海陽子，小山時隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 維管束組織の細胞時計と光周性花成応答 (2014)
  • Author(s): 伊藤照悟
  • Organizer: 京大植物縦横無尽の会 ワークショップ
  • Place of Presentation: 京都大学 京都
  • Year and Date: 2014-11-14
• [Presentation] Day-length sensing mechanisms regulating the timing of the floral transition in Arabidopsis leaf vasculature (2014)
  • Author(s): Shogo Ito
  • Organizer: Bristol-Kyoto Workshop on Plant Environmental Signalling
  • Place of Presentation: Bristol University UK
  • Year and Date: 2014-09-22 – 2014-09-25
• [Presentation] Identification of Transcriptional Regulators Controlling CONSTANS Expression for Photoperiodic Flowering in Arabidopsis (2014)
  • Author(s): 伊藤照悟、Golembeski Greg、Kwon Micheal、Breton Ghislain、小山知嗣、高木 優、Kay Steve A.、今泉貴登
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山県 富山大学 五福キャンパス
• [Remarks] Oyama Lab
  • URL: http://cosmos.bot.kyoto-u.ac.jp/clock/index.html