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Functional analysis of protein kinase that interacts with blue light-receptor phototropin in stomatal opening

Research Project

Project/Area Number 25840105
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

INOUE Shin-ichiro  名古屋大学, 理学(系)研究科(研究院), 助教 (40532693)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords気孔 / 青色光 / フォトトロピン / プロテインキナーゼ / 細胞膜プロトンポンプ
Outline of Final Research Achievements

Stomata in the plant epidermis open in response to blue light and contribute to plant growth by regulating photosynthesis and transpiration. A blue light-receptor phototropin mediates the stomatal opening via promotion of an active transport of K+ in stomatal guard cells. However, signaling for the stomatal opening is not fully understood. In this study, we performed functional analysis of a protein kinase that interacts with phototropin, and characterized roles of the protein kinase in the blue light signaling for stomatal opening.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Invited: 2 results)

  • [Journal Article] Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells.2015

    • Author(s)
      Sakai Y, Inoue S, Harada A, Shimazaki K, Takagi S
    • Journal Title

      Journal of Integrative Plant Biology

      Volume: 57 Pages: 93-105

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mg-chelatase I subunit 1 and Mg-Protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana.2014

    • Author(s)
      Tomiyama M, Inoue S, Tsuzuki T, Soda M, Morimoto S, Okigaki Y, Ohishi T, Mochizuki N, Takahashi K, Kinoshita T
    • Journal Title

      Journal of Plant Research

      Volume: 127 Pages: 553-563

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mg-chelatase I subunit 1 and Mg-Protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana.2014

    • Author(s)
      Tomiyama M, Inoue S , Tsuzuki T, Soda M, Morimoto S, Okigaki Y, Ohishi T, Mochizuki N, Takahashi K, Kinoshita T
    • Journal Title

      Journal of Plant Research

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Abscisic acid suppresses hypocotyl elongation by dephosphorylating Plasma membrane H^+-ATPase in Arabidopsis thaliana2014

    • Author(s)
      Hayashi Y, Takahashi K, Inoue S, Klnoshita T
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Issue: 4 Pages: 845-853

    • DOI

      10.1093/pcp/pcu028

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth.2014

    • Author(s)
      Wang Y, Noguchi K, Ono N, Inoue S, Terashima I, Kinoshita T
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111 Issue: 1 Pages: 533-538

    • DOI

      10.1073/pnas.1305438111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana.2013

    • Author(s)
      Tsuzuki T, Takahashi K, Tomiyama M, Inoue S, Kinoshita T
    • Journal Title

      Frontiers in Plant Science

      Volume: 4 Pages: 440-440

    • DOI

      10.3389/fpls.2013.00440

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsisthahana.2013

    • Author(s)
      Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T
    • Journal Title

      Plant Physiology

      Volume: 162 Issue: 3 Pages: 1529-38

    • DOI

      10.1104/pp.113.217984

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] New insight into blue light-induced stomatal opening in Arabidopsis2015

    • Author(s)
      井上晋一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物の青色光受容体フォトトロピンのリン酸化反応を介したシグナル伝達機構2014

    • Author(s)
      井上晋一郎
    • Organizer
      第18回日本光生物学協会年会
    • Place of Presentation
      大阪市立大学学術情報総合センター
    • Year and Date
      2014-08-22 – 2014-08-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CIPK23 positively regulates blue-light-induced stomatal opening in Arabidopsis2014

    • Author(s)
      Inoue S, Kaiserli E, Takahashi H, Tomokiyo Y, Kinoshita T, Christie JM, Sawasaki T, Shimazaki K
    • Organizer
      Gordon research conference "Photosensory Receptors & Signal Transduction"
    • Place of Presentation
      Il Ciocco Lucca (Barga), Italy
    • Year and Date
      2014-04-06 – 2014-04-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物の青色光受容体フォトトロピンのリン酸化反応を介したシグナル伝達2014

    • Author(s)
      井上晋一郎
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー」地区ミニシンポジウム
    • Place of Presentation
      東北大学、宮城、日本
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Phototropin-dependent phosphorylation of a plasma membrane syntaxin SYP132 in Arabidopsis2013

    • Author(s)
      Inoue S, Tomokiyo Y, Kinoshita T, Shimazaki K
    • Organizer
      International Symposium on Plant Photobiology (ISPP) 2013
    • Place of Presentation
      Edinburgh University, Scotland, UK
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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