| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Very few genes required for plant fertilization are known and to understand the molecular mechanism of it, I need to obtain the factors required for plant fertilization. To obtain the factors, I started a screening by using a very effective 'vanillin staining method' to distinguish fertilized ovules from unfertilized ovules. I have already screened 13698 plants and identified 382 mutants. Of these mutants, I observed and checked 9 mutants and identified 7 female gametophytic mutants and 2 male gametophytic mutants. It is clear that the 'vanillin staining method' is very efficient to identify mutants defective in plant fertilization.
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