Identification of factors required for plant fertilization by using a new phenomenon, fertilization independent ovule enlargement.
| Project/Area Number |
25840106
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
KASAHARA Ryushiro 名古屋大学, トランスフォーマティブ生命分子研究所, 招へい教員 (40467270)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物の受精 / 受精不全変異体 / バニリン染色 / 受精非依存的種子肥大 / 雌性配偶体 / 雄性配偶体 / 受精因子 / 品種改良 / 受精欠損 / スクリーニング / 種子肥大 / 部分的種皮形成 / 変異体 / 遺伝子同定 |
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| Outline of Final Research Achievements |
Very few genes required for plant fertilization are known and to understand the molecular mechanism of it, I need to obtain the factors required for plant fertilization. To obtain the factors, I started a screening by using a very effective 'vanillin staining method' to distinguish fertilized ovules from unfertilized ovules. I have already screened 13698 plants and identified 382 mutants. Of these mutants, I observed and checked 9 mutants and identified 7 female gametophytic mutants and 2 male gametophytic mutants. It is clear that the 'vanillin staining method' is very efficient to identify mutants defective in plant fertilization.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] 植物の受精学2015
Author(s)
笠原竜四郎
Organizer
静岡大学理学部 集中講義
Place of Presentation
静岡大学 静岡
Year and Date
2015-10-17
Related Report
Invited
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