Identification of LRR-RKs involved in perception of root meristem growth factor

• Project/Area Number: 25840111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University (2014-2016); National Institute for Basic Biology (2013)
• Principal Investigator: Shinohara Hidefumi 名古屋大学, 理学研究科, 助教 (40547022)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物 / ペプチドホルモン / 受容体キナーゼ / 根端メリステム / 硫酸化ペプチド / ペプチドシグナル / リガンド－受容体ペア / 細胞間情報伝達 / リガンド受容体ペア

Outline of Final Research Achievements

A peptide hormone, root meristem growth factor (RGF), regulates root meristem development through the PLETHORA stem cell transcription factor pathway, but it remains to be uncovered how extracellular RGF signals are transduced to the nucleus. We identified, using a receptor kinase expression library and photoaffinity labeling, three receptor kinases that directly interact with RGF peptides in Arabidopsis. The triple rgfr mutant was insensitive to externally applied RGF peptide and displayed a short root phenotype accompanied by a considerable decrease in meristematic cell number. In addition, PLT1 and PLT2 protein gradients, observed as a gradual gradient decreasing toward the elongation zone from the stem cell area in wild type, steeply declined at the root tip in the triple mutant. Our results strongly suggest that RGFRs mediate the transformation of an RGF peptide gradient into a PLT protein gradient in the meristem, thereby acting as key regulators of root meristem development.

Research Products

• [Journal Article] A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots (2017)
  • Author(s): Nakayama T, Shinohara H, Tanaka M, Baba K, Ogawa-Ohnishi M, Matsubayashi Y.
  • Journal Title: Science Volume: 355 Issue: 6322 Pages: 284-286
  • DOI: 10.1126/science.aai9057
  • NAID: 120006024333
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cryptic bioactivity capacitated by synthetic hybrid plant peptides (2017)
  • Author(s): Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU, Uchida N.
  • Journal Title: Nature Commun. Volume: 8 Issue: 1 Pages: 14318-14318
  • DOI: 10.1038/ncomms14318
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. (2016)
  • Author(s): Shinohara H, Mori A, Yasue N, Sumida K and Matsubayashi Y.
  • Journal Title: Proc. Natl. Acad. Sci. U S A Volume: 印刷中 Issue: 14 Pages: 3897-3902
  • DOI: 10.1073/pnas.1522639113
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view (2015)
  • Author(s): Shinohara H and Matsubayashi Y
  • Journal Title: Plant J. Volume: 82 Issue: 2 Pages: 328-336
  • DOI: 10.1111/tpj.12817
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Perception of root-derived peptides by shoot LRR-RKs mediates systemic N-demand signaling. (2014)
  • Author(s): Tabata R, Sumida K, Yoshii T, Ohyama K, Shinohara H, Matsubayashi Y
  • Journal Title: Science Volume: 346 Issue: 6207 Pages: 343-346
  • DOI: 10.1126/science.1257800
  • Peer Reviewed
• [Journal Article] Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase. (2013)
  • Author(s): Okamoto, S., Shinohara, H., Mori, T., Matsubayashi, Y., and Kawaguchi, M.
  • Journal Title: Nat. Commun. Volume: 4 Issue: 1 Pages: 2191-2191
  • DOI: 10.1038/ncomms3191
  • Peer Reviewed
• [Journal Article] A novel pollen-pistil interaction conferring high temperature tolerance during reproduction via CLE45 signaling. (2013)
  • Author(s): Endo, S., Shinohara, H., Matsubayashi, Y., and Fukuda, H.
  • Journal Title: Curr Biol. Volume: 23 Issue: 17 Pages: 1670-1676
  • DOI: 10.1016/j.cub.2013.06.060
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] RGF情報伝達系の解明に向けたケミカルスクリーニング (2017)
  • Author(s): 篠原秀文，林陽子，松林嘉克
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学，鹿児島
  • Year and Date: 2017-03-16
• [Presentation] 根端メリステムの活性を制御するペプチドホルモンRGFの受容体の同定 (2016)
  • Author(s): 篠原秀文，林陽子，松林嘉克
  • Organizer: 植物化学調節学会第51回大会
  • Place of Presentation: 高知大学，高知
  • Year and Date: 2016-10-28
• [Presentation] Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view (2015)
  • Author(s): Hidefumi Shinohara
  • Organizer: 3rd European Workshop on Peptide Signalling and Activity in Plants
  • Place of Presentation: Ghent, Belgium
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] PCP論文賞受賞講演 (2015)
  • Author(s): 篠原秀文
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 受容体キナーゼ発現ライブラリーを用いたRGF受容体の同定 (2014)
  • Author(s): 篠原秀文，松林嘉克
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学，富山
• [Presentation] アラビノシル化CLV3ペプチドはBAM1に結合する (2013)
  • Author(s): 篠原秀文，松林嘉克
  • Organizer: 植物化学調節学会第48回大会
  • Place of Presentation: 新潟大学，新潟
• [Book] CSJカレントレビュー19 生物活性分子のケミカルバイオロジー (2015)
  • Author(s): 松林嘉克，篠原秀文
  • Total Pages: 7
  • Publisher: 化学同人
• [Remarks] 名古屋大学大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ
  • URL: http://www.bio.nagoya-u.ac.jp/~b2/index.html
• [Remarks] 名古屋大学大学院理学研究科生命理学専攻細胞間シグナル研究グループ
  • URL: http://www.bio.nagoya-u.ac.jp/~b2/index.html
• [Remarks] 名古屋大学大学院理学研究科生命理学専攻細胞間シグナル研究グループ
  • URL: http://www.bio.nagoya-u.ac.jp/~b2/
• [Remarks] 名古屋大学大学院理学研究科生命理学専攻細胞間シグナル研究グループ
  • URL: http://www.bio.nagoya-u.ac.jp/~b2/index.html