| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Arabidopsis transgenic plant (EC-PM-GFP), where the egg cell plasma membrane is specifically labeled with GFP, was produced. The protein extract derived from collected pistils were subjected to immnoprecipitation using anti-GFP antibody. As a result, specific proteins such as GFP were successfully identified only in the proteins from transgenic pistils, but not in those of wild type pistils. It was indicated that egg cell proteins within the pistil cell population were successfully isolated by our method. This method has a great advantage of avoiding laborious work to handle the egg cell.
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