Study on functional evolution of the digestive hormone peptide cholecystokinin(CCK)/gastrin: Expression analysis of CCK/gastrin peptide in ascidian Ciona intestinalis

• Project/Area Number: 25840114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Kanazawa University
• Principal Investigator: TOSHIO Sekiguchi 金沢大学, 環日本海域環境研究センター, 助教 (40378568)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: コレシストキニン / ガストリン / カタユウレイボヤ / Cholecystokinin / gastrin / ciona intestinalis / cionin

Outline of Final Research Achievements

To elucidate the evolution of the digestive hormone cholecystokinin (CCK)/gastrin in chordates, we analyzed the expression of cionin which is a CCK/gastrin ortholog and cionin receptor in the ascidian Ciona intestinalis. Analysis of cionin receptor gene promoter-driven fluorescence demonstrated that the cionin receptor was expressed in the visceral ganglion and tail nerve cord of larval ascidians. This expression pattern coincided with cholinergic neurons. In adult ascidians, cionin mRNA was exclusively expressed in the central nervous system, whereas the expression of cionin receptor mRNA was detected in both the central nervous system and digestive tract. These results suggest that cionin is released from the central nervous system and acts as a neurotransmitter or neuromodulator, affecting cionin receptor-expressing neurons. Alternatively, cionin can affect the digestive tract as a digestive hormone.

Research Products

• [Journal Article] Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone (2014)
  • Author(s): Yachiguchi K, Sekiguchi T, Nakano M, Hattori A, Yamamoto M, Kitamura K, Maeda M, Tabuchi Y, Kondo T, Kamauchi H, Nakabayashi H, Srivastav AK, Hayakawa K, Sakamoto T, Suzuki N
  • Journal Title: Zoolog Sci Volume: 31 Issue: 5 Pages: 330-337
  • DOI: 10.2108/zs130265
  • NAID: 120005463340
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased PGE2 has a positive correlation with plasma calcium during goldfish reproduction. (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimazaki, Y., Sekiguchi, T., Kamauchi, H., Mishima, H., Wada, S., Srivastav, A.K., Hayakawa, K. and Oshima, Y.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 59 Pages: 97-101
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales (2013)
  • Author(s): Yano S, et al. (14番目)
  • Journal Title: Zoological Science Volume: 30 Issue: 3 Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Accumulation of organotins in wharf roach (Ligia exotica Roux) and its ability to serve as a biomonitoring species for coastal pollution (2013)
  • Author(s): Undap S.L., Matsunaga, S., Honda, M., Sekiguchi, T., Suzuki, N., Khalil, F., Qiu, X., Shimasaki, Y., Ando, H., Sato-Okoshi, W., Sunobe, T., Takeda, S., Munehara, H., Oshima, Y.
  • Journal Title: Ecotoxicology and Environmental Safety Volume: 96 Pages: 75-79
  • DOI: 10.1016/j.ecoenv.2013.06.019
  • Peer Reviewed
• [Journal Article] Insight into Molecular and Functional Diversity of Tachykinins and their Receptors. (2013)
  • Author(s): Satake, H., Aoyama, M., Sekiguchi, T., Kawada, T.
  • Journal Title: Protein & Peptide Letters Volume: 20 Issue: 6 Pages: 615-627
  • DOI: 10.2174/0929866511320060002
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., et al.
  • Journal Title: Env. Sci. Poll. Res. Volume: 12 Issue: 10 Pages: 1347-1355
  • DOI: 10.1007/s11356-012-1347-5
  • NAID: 120005253213
  • Peer Reviewed / Open Access
• [Presentation] カタユウレイボヤにおけるcioninおよび受容体の発現解析． (2014)
  • Author(s): 谷口詩穂，小笠原道生，佐竹 炎，鈴木信雄，関口俊男
  • Organizer: 平成26年度日本動物学会中部支部大会
  • Place of Presentation: のと勤労者プラザ、石川県
  • Year and Date: 2014-11-23 – 2014-11-24
• [Presentation] カルシウム調節ホルモンの分子進化． (2014)
  • Author(s): 関口俊男
  • Organizer: 平成26年度日本動物学会中部支部大会
  • Place of Presentation: のと勤労者プラザ，石川県
  • Year and Date: 2014-11-23 – 2014-11-24
  • Invited
• [Presentation] カタユウレイボヤを用いた内分泌系進化の研究． (2014)
  • Author(s): 関口俊男
  • Organizer: 日本動物学会第85回仙台大会 関連集会ホヤの談話会
  • Place of Presentation: 東北大学、宮城県
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] 脊椎動物カルシトニンの起源と多様化の研究． (2014)
  • Author(s): 関口俊男
  • Organizer: 全国国立大学臨海臨湖実験所所長会議シンポジウム「海産動物の生体制御学的研究とその拡がり」
  • Place of Presentation: 岡山大学理学部附属牛窓臨海実験所、岡山県
  • Year and Date: 2014-06-05
  • Invited
• [Presentation] 円口類のカルシトニンとカルシトニン受容体についての研究. (2013)
  • Author(s): 関口俊男，上西篤志，山本 樹，谷内口孝治，鈴木信雄
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山
• [Presentation] 新規メラトニン誘導体の卵巣摘出ラットに対する作用． (2013)
  • Author(s): (18) 関口俊男，帖地 藍，関あずさ，高垣裕子，池亀美華，田渕圭章，近藤 隆，北村敬一郎，清水宣明，矢野幸子，服部淳彦，鈴木信雄
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎
• [Book] Gastrin family. in “Handbook on Hormones”. Y. Takei, H. Ando, and K.Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi T.
  • Publisher: ELSEVIER
• [Book] Gastrin. In “Handbook on Hormones”. Y. Takei, H. Ando, and K.Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi T.
  • Publisher: ELSEVIER
• [Book] Cholecystokinin. In “Handbook on Hormones”. Y. Takei, H. Ando, and K.Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi T.
  • Publisher: ELSEVIER
• [Book] Caerulein. In “Handbook on Hormones”. Y. Takei, H. Ando, and K.Tsutsui. Eds. (2015)
  • Author(s): Sekiguchi T.
  • Publisher: ELSEVIER
• [Remarks] 金沢大学臨海実験施設
  • URL: http://rinkai.w3.kanazawa-u.ac.jp/
• [Remarks] 金沢大学環日本海域環境研究センター臨海実験施設
  • URL: http://rinkai.w3.kanazawa-u.ac.jp/