Regulatory mechanism of hypothalamus-pituitary system as a basis of vertebrate's evolution

• Project/Area Number: 25840115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Shizuoka University
• Principal Investigator: OKADA Reiko 静岡大学, 理学部, 講師 (50386554)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 視床下部 / 下垂体 / 両生類 / 環境適応 / 温度 / 内分泌

Outline of Final Research Achievements

Hypothalamus-pituitary-thyroid gland (HPT) axis plays an important role to maintain body temperature in mammals. Thyrotropin-releasing factor (TRH) is a major factor that enhances the release of thyroid-stimulating hormone (TSH) from the pituitary in mammals, whereas it mainly acts as a prolactin-releasing factor in amphibians. In this study, it was revealed that expression of TRH precursor mRNA in the frog hypothalamus was increased by cold stimulation as in mammals. The discrepancy in the role of hypothalamic factors between amphibians and mammals is discussed in relation to metamorphosis and thermoregulation.

Research Products

• [Journal Article] Morphological, biochemical, transcriptional and epigenetic responses to fasting and refeeding in intestine of Xenopus laevis. (2016)
  • Author(s): Tamaoki, K., Okada, R., Ishihara, A., Shiojiri, N., Mochizuki, K., Goda, T., Yamauchi, K.
  • Journal Title: Cell Biosci. Volume: 6 Issue: 1 Pages: 2-2
  • DOI: 10.1186/s13578-016-0067-9
  • NAID: 120005698530
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 両生類の変態：分子から個体レベルの制御 (2016)
  • Author(s): 岡田令子、鈴木賢一
  • Journal Title: 生物科学 Volume: 未定
  • Peer Reviewed
• [Journal Article] Molecular machinery for vasotocin-dependent transepithelial water movement in amphibians: aquaporins and evolution. (2015)
  • Author(s): Suzuki, M., Shibata, Y., Ogushi, Y., Okada, R.
  • Journal Title: Biol. Bull. Volume: 229 Pages: 109-119
  • Peer Reviewed
• [Journal Article] Molecular cloning of cDNA encoding an aquaglyceroporin, AQP-h9, in the Japanese tree frog, Hyla japonica: possible roles of AQP-h9 as a glycerol transporter in freeze tolerance (2015)
  • Author(s): Hirota A, Takiya Y, Sakamoto J, Shiojiri N, Suzuki M, Tanaka S, Okada R.
  • Journal Title: Zoolog Sci. Volume: 32 Issue: 3 Pages: 296-306
  • DOI: 10.2108/zs140246
  • Peer Reviewed
• [Journal Article] Molecular cloning of cDNA encoding an aquaglyceroporin, AQP-h9, in the Japanese tree frog, Hyla japonica: possible roles of AQP-h9 in freeze tolerance. (2015)
  • Author(s): Hirota, A., Takiya, Y., Sakamoto, J., Shiojiri, N., Suzuki, M., Tanaka, S., Okada., R.
  • Journal Title: Zool Sci Volume: 32 Pages: 296-306
  • NAID: 120005775043
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular and cellular characterization of urinary bladder-type aquaporin in Xenopus laevis (2014)
  • Author(s): Yuki Shibata, Izumi Katayama, Yuji Ogushi, Takashi Nakakura, Reiko Okada, Shigeyasu Tanaka, Masakazu Suzuki.
  • Journal Title: Gen Comp Endocrinol. Volume: 222 Pages: 11
  • DOI: 10.1016/j.ygcen.2014.09.001
  • URL: https://pure.teikyo.jp/en/publications/ebee320d-4860-43d8-8d2f-dc47031c40fb
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gene expression and localization of two types of AQP5 in Xenopus tropicalis under hydration and dehydration. (2014)
  • Author(s): Yuki Shibata, Junya Hara, Takahiro Sano, Masakazu Suzuki, Reiko Okada, Hiroshi Mochida, and Shigeyasu Tanaka.
  • Journal Title: Am J Physiol Regul lntegr Comp Physiol Volume: (In press) Issue: 1 Pages: R44-R56
  • DOI: 10.1152/ajpregu.00186.2013
  • Peer Reviewed
• [Journal Article] Novel vasotocin-regulated aquaporins expressed in the ventral skin of semigaquatic anuran amphibians: evolution of cutaneous water-absorbing mechanisms. (2014)
  • Author(s): Saitoh, Y., Ogushi, Y., Shibata, Y., Okada, R., Tanaka, S., Suzuki, M
  • Journal Title: Endocrinology Volume: in press Issue: 6 Pages: 2166-2177
  • DOI: 10.1210/en.2013-1928
  • Peer Reviewed
• [Journal Article] Tanaka S, Nakakura T, Jansen EJ, Unno K, Okada R, Suzuki M, Martens GJ, Kikuyama S. (2013)
  • Author(s): Angiogenesis in the intermediate lobe of the pituitary gland alters its structure and function.
  • Journal Title: Gen Comp Endocrinol Volume: in press Pages: 10-18
  • DOI: 10.1016/j.ygcen.2013.01.009
  • Peer Reviewed
• [Presentation] Regulation: Hypothalamic control of the release of thyrotropin and corticotropin in amphibians. (2015)
  • Author(s): Reiko Okada
  • Organizer: 第40回日本比較内分泌学会大会日本比較生理生化学会第37会大会合同大会
  • Place of Presentation: アステールプラザ（広島県広島市）
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] 両生類から見た脊椎動物間における下垂体調節因子の使いまわし (2015)
  • Author(s): 岡田令子
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] ニホンアマガエル凍結時におけるGLUTの発現 (2015)
  • Author(s): 阿達駿，滝谷優，廣田敦司，鈴木雅一，岡田令子
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] ウシガエル下垂体前葉におけるPOMC mRNAの発現に及ぼすAVTの影響 (2014)
  • Author(s): 四元優太郎，山本和俊，菊山榮，岡田令子
  • Organizer: 平成26年度日本動物学会中部支部大会
  • Place of Presentation: 石川県鳳珠郡能登町 ホテルのときんぷら
  • Year and Date: 2014-11-22 – 2014-11-24
• [Presentation] Aquaporins involve in freeze-tolerance in hibernating Japanese green tree frog, Hyla japonica. (2014)
  • Author(s): Hirota, A., Takiya, Y., Suzuki, M., Shiojiri, N., Tanaka, S., Okada, R.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Elevation of thyrotropin-releasing hormone precursor mRNA expression in the amphibian brain in response to cold temperature. (2014)
  • Author(s): Kowata, N., Nakano, M., Kobayashi, T., Hasunuma, I., Yamamoto, K., Kikuyama, S., Okada, R.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Molecular evolution and physiological roles of aquaporins expressed in anuran osmoregulatory organs. (2014)
  • Author(s): Suzuki, M., Shibata, Y., Okada, R.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
  • Invited
• [Presentation] Arginine vasotocin receptors in the amphibian brain and pituitary. (2014)
  • Author(s): Hasunuma, I., Toyoda, F., Okada, R., Yamamoto, K., Iwamuro, S., Kikuyama, S.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
  • Invited
• [Presentation] Angiogenesis in the pars intermedia of the pituitary alters its structure and function. (2014)
  • Author(s): Nakakura, T., Unnno, K., Okada, R., Suzuki, M., Jansen, E.J.R., Martens, G.J.M., Kikuyama, S., Tanaka, S.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Spatial and temporal expression of aquaporins, AQP2 and AQP3, in the kidney during metamorphosis of the tree frog, Hyla japonica. (2014)
  • Author(s): Ueno, T., Shibata, Y., Sano, H., Yamashita, S., Okada, R., Suzuki, M.
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] 凍結時のアマガエル肝臓におけるGLUT2の発現 (2014)
  • Author(s): 岡田令子，廣田敦司，滝谷優，鈴木雅一，田中滋康
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 宮城県仙台市 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] アマガエル凍結時のグリセロールおよびアクアグリセロポリンの機能 (2014)
  • Author(s): 廣田敦司，滝谷優，鈴木雅一，塩尻信義，田中滋康，岡田令子
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 宮城県仙台市 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 有尾両生類における腹側皮膚型アクアポリン（AQPa2S）の機能と分子進化 (2014)
  • Author(s): 柴田侑毅，Hillyard, S.D.，岡田令子，田中滋康，鈴木雅一，長井孝紀
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 宮城県仙台市 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] アフリカツメガエルを用いた消化管の絶食／再摂食に対する応答の検討 (2014)
  • Author(s): 玉置啓二，岡田令子，石原顕紀，望月和樹，山内清志
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 宮城県仙台市 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 有尾両生類に発現する両生類特異的なAVT調節性アクアポリン（AQP）の機能と分子進化 (2014)
  • Author(s): 柴田侑毅，Stanley D Hillyard，岡田令子，田中滋康，鈴木雅一，長井孝紀
  • Organizer: 平成25年度日本動物学会中部支部大会
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター（愛知県）
• [Presentation] ウシガエルTRH前駆体mRNA発現に及ぼす環境温度の影響 (2013)
  • Author(s): 木幡奈都乃，中野真樹，小林哲也，蓮沼至，山本和俊，菊山榮，岡田令子
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県）
• [Presentation] ネッタイツメガルの腹側皮膚型アクアポリン（AQP）を介した極限水環境への順応機構 (2013)
  • Author(s): 柴田侑毅，佐野貴大，岡田令子，鈴木雅一，田中滋康
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] Molecular evolution and function of the orthologue of mammalian aquaporin 5 in anuran and urodele amphibians. (2013)
  • Author(s): Shibata, Y., Hillyard, S.D., Suzuki, M., Okada, R., Nagai, T., Tanaka, S.
  • Organizer: 17th International Congress of Comparative Endocrinology
  • Place of Presentation: Universitat de Barcelona (Barcelona, Spain)