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Elucidation of epigenetic mechanism that regulates a neural circuit for C. elegans behavioral plasticity

Research Project

Project/Area Number 25840124
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genetics/Chromosome dynamics
Research InstitutionKyoto University

Principal Investigator

SUGI Takuma  京都大学, 物質-細胞統合システム拠点, 助教(特任) (70571305)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords記憶 / エピジェネティクス / 線虫C. elegans / 線虫
Outline of Final Research Achievements

In this study, I investigated a mechanistic relationship between epigenetics and behavioral memory in C. elegans. I initially developed a multiplexed optical system to genetically analyzed C. elegans mechanosensory habituation in a high-throughput manner. Then, using this system, I identified two interneurons involved in memory formation, and an epigenetic factor important for memory formation.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 3 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Simplified method for cell-specific gene expression analysis in Caenorhabditis elegans.2014

    • Author(s)
      Sugi T, Ohtani Y
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 450 Issue: 1 Pages: 330-334

    • DOI

      10.1016/j.bbrc.2014.05.124

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-throughput optical quantification of mechanosensory habituation reveals neurons encoding memory in Caenorhabditis elegans.2014

    • Author(s)
      Sugi T, Ohtani Y, Kumiya Y, Igarashi R, Shirakawa M
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 111 Issue: 48 Pages: 17236-17241

    • DOI

      10.1073/pnas.1414867111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Versatile strategy for isolating TALEN-mediated knockout mutants in Caenorhabditis elegans.2014

    • Author(s)
      Sugi T, Sakuma T, Ohtani T and Yamamoto T
    • Journal Title

      Dev Growth Differ

      Volume: 56 Issue: 1 Pages: 78-85

    • DOI

      10.1111/dgd.12108

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Regulation of gene expression and behavior by genome editing technology in C. elegans2014

    • Author(s)
      Takuma Sugi
    • Organizer
      MicroRNAs & Single Molecule Biology/Genome Editing Europe Symposia-2014
    • Place of Presentation
      University of Cambridge
    • Year and Date
      2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-throughput behavioral imaging reveals the neurons responsible for mechanosensory memory in C. elegans

    • Author(s)
      Takuma Sugi
    • Organizer
      19th international C. elegans meeting
    • Place of Presentation
      Los Angeles
    • Related Report
      2013 Research-status Report
  • [Presentation] Biophysical analysis of C. elegans mechanosensory learning and memory

    • Author(s)
      Takuma Sugi
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 線虫C. elegansのゲノム編集による個体機能の再構成への試み

    • Author(s)
      杉 拓磨
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 線虫の記憶を刻むリズムとエピジェネティクス

    • Author(s)
      杉 拓磨
    • Organizer
      理研エピジェネティクスセミナーシリーズ
    • Place of Presentation
      理研(和光)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Targeted Genome Editing Using Site-specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System2014

    • Author(s)
      Takuma Sugi(分担執筆)
    • Total Pages
      205
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 嫌いな刺激に馴れる仕組みを線虫で発見

    • URL

      http://www.icems.kyoto-u.ac.jp/j/pr/2014/11/18-nr.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 嫌いな刺激に馴れる仕組みを線虫で発見

    • URL

      http://www.jst.go.jp/pr/announce/20141118/

    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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