Evolutionary developmental study of the cyclostome towards acquisition of the paired nostrils.

• Project/Area Number: 25840133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Hyogo Medical University
• Principal Investigator: Sugahara Fumiaki 兵庫医科大学, 医学部, 講師 (00611005)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 円口類 / ヤツメウナギ / ヌタウナギ / 鼻下垂体プラコード / 顔面形成 / 脊椎動物 / 形態進化 / 鼻 / 下垂体 / プラコード / 進化発生学 / 鼻プラコード / 下垂体プラコード / 細胞系譜

Outline of Final Research Achievements

First, we observed the morphological development of the naso-hypophyseal placode (NHP) in lampreys by using a confocal microscope and a scanning electron microscope. We found that the placode thickening was seen only in the nasal area and not in the ectoderm ventral to the hypothalamus where the hypophyseal placode is formed in gnathostomes. Previous reports suggested that lamprey NHP is initially distant from the hypothalamus, grows caudally and attaches secondarily to the hypothalamus. However, our gene expression analysis and DiI labeling experiments have shown that the hypophyseal placode cells are not supplied by migration from the nasal area. Next, we tried DiI labeling to trace the presumptive nasal placode cells in the pre-placodal region (PPR). This experiment has been successfully done and the analysis is in progress. Finally, we successfully got a few hagfish embryos and made histological sections for in situ hybridizations.

Research Products

• [Journal Article] Evidence from cyclostomes for complex regionalization of the ancestral vertebrate brain (2016)
  • Author(s): Fumiaki Sugahara, Juan Pascual-Anaya, Yasuhiro Oisi, Shigehiro Kuraku, Shin-IchiAota, Noritaka Adachi, Wataru Takagi, Tamami Hirai, Noboru Sato, Yasunori Murakami, Shigeru Kuratani
  • Journal Title: Nature Volume: 531 Issue: 7592 Pages: 97-100
  • DOI: 10.1038/nature16518
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reevaluating Emx gene phylogeny: homopolymeric amino acid tracts as a potential factor obscuring orthology signals in cyclostome genes. (2015)
  • Author(s): Noro, M., Sugahara, F. and Kuraku, S.
  • Journal Title: BMC Evolutionary Biology Volume: 15 Issue: 1 Pages: 78-78
  • DOI: 10.1186/s12862-015-0351-z
  • Peer Reviewed / Open Access
• [Journal Article] Evolution of the regionalization and patterning of the vertebrate telencephalon: what can we learn from cyclostomes? (2013)
  • Author(s): Fumiaki Sugahara
  • Journal Title: Current Opinion in Genetics & Development Volume: 23 Issue: 4 Pages: 475-483
  • DOI: 10.1016/j.gde.2013.02.008
  • Peer Reviewed
• [Presentation] Brain development of the hagfish; the origin of complex brain compartmentalization of the vertebrates (2015)
  • Author(s): Sagahara Fumiaki, Oisi Yasuhiro, Pascual-Anaya Juan, Kuraku Shigehiro, Aota Shin-ichi, Adachi Noritaka, Hirai Tamami, Murakami Yasunori, Kuratani Shigeru
  • Organizer: Inaugural 2015 meeting of the Pan-American Society for Evolutionary Developmental Biology
  • Place of Presentation: Berkeley (U.S.A)
  • Year and Date: 2015-08-05
  • Int'l Joint Research
• [Presentation] Dorso-Ventral Patterning of the Lamprey Telencephalon by Foxg1, Evolution of the Vertebrate Telencephalon (2015)
  • Author(s): Sugahara Fumiaki, Aota Shin-ichi, Murakami Yasunori, Sato Noboru, Kuratani Shigeru
  • Organizer: 48h Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際会議場 (茨城県つくば市)
  • Year and Date: 2015-06-02
• [Presentation] The deep roots of vertebrate genomic evolution: insights from the embryonic transcriptome and Hox genes of the hagfish (2015)
  • Author(s): Pascual-Anaya Juan, Perez-Pulido Antonio, Sugahara Fumiaki, Kuratani Shigeru
  • Organizer: 48th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際会議場 (茨城県つくば市)
  • Year and Date: 2015-06-02
• [Presentation] Regionalization of the Lamprey Telencephalon by Foxg1; Evolution of DV Patterning of the Vertebrate Telencephalon. (2015)
  • Author(s): Sugahara Fumiaki, Aota Shin-ichi, Murakami Yasunori, Onai Takayuki, Sato Noboru, Kuratani Shigeru
  • Organizer: 120th Annual Meeting of The Japanese Association of Anatomists and the 92th Annual Meeing of The Physiological Society of Japan
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 円口類ヤツメウナギにおける鼻下垂体プラコードの発生から探る、脊椎動物の対鼻性獲得の過程 (2014)
  • Author(s): 菅原文昭
  • Organizer: 日本進化学会第16回大阪大会
  • Place of Presentation: 高槻
  • Year and Date: 2014-08-21 – 2014-08-23
• [Presentation] 円口類ヤツメウナギにおける鼻下垂体プラコード(NHP)の発生からみた脊椎動物顔面形態の進化 (2014)
  • Author(s): 菅原 文昭
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木・下野
• [Book] In Situ Hybridization Methods (Neuromethods) (2015)
  • Author(s): Fumiaki Sugahara, Yasunori Murakami and Shigeru Kuratani
  • Total Pages: 592
  • Publisher: Human Press
• [Book] Neuromethods (2014)
  • Author(s): Fumiaki Sugahara, Yasunori Murakami and Shigeru Kuratani
  • Publisher: Springer
• [Remarks] 円口類から解き明かされる脳の領域化の進化的な起源
  • URL: http://first.lifesciencedb.jp/archives/12168
• [Remarks] 脳の進化的起源を解明
  • URL: http://www.riken.jp/pr/press/2016/20160216_1/