| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
First, we observed the morphological development of the naso-hypophyseal placode (NHP) in lampreys by using a confocal microscope and a scanning electron microscope. We found that the placode thickening was seen only in the nasal area and not in the ectoderm ventral to the hypothalamus where the hypophyseal placode is formed in gnathostomes. Previous reports suggested that lamprey NHP is initially distant from the hypothalamus, grows caudally and attaches secondarily to the hypothalamus. However, our gene expression analysis and DiI labeling experiments have shown that the hypophyseal placode cells are not supplied by migration from the nasal area. Next, we tried DiI labeling to trace the presumptive nasal placode cells in the pre-placodal region (PPR). This experiment has been successfully done and the analysis is in progress. Finally, we successfully got a few hagfish embryos and made histological sections for in situ hybridizations.
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