• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evolutional study on the chytrids based on life cycle and infection manner

Research Project

Project/Area Number 25840135
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionThe University of Tokyo

Principal Investigator

Ota Shuhei  東京大学, 新領域創成科学研究科, 特任助教 (20455926)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsツボカビ / レクチン / 生活環 / 進化 / 藻類
Outline of Final Research Achievements

This study aimed to reveal the evolution and fungi-algae interaction based on the detailed observation of life cycle and manner of infection in chytrids, a primitive group of fungi. Chytrid ultrastructure was observed by transmission electron microscopy. Time-lapse microscopy was performed to observe their transition of life cycle stage, and fluorescent microscopy was performed to examine lectin-binding pattern in each life cycle stage. As a result, the lectin-binding patterns and the timing of zoospore release were different among life cycle stages or chytrid species. It was suggested that N-acetyl-D-glucosamine, one of the amino-sugars, was accumulated highly at the basal part of the rhizoid system.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (44 results)

All 2016 2015 2014 2013 Other

All Journal Article (19 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (25 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.2016

    • Author(s)
      Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bisova, K., Zachleder, V., Kawano, S.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 25731-25731

    • DOI

      10.1038/srep25731

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure2016

    • Author(s)
      Shuhei Ota, Kenshiro Oshima, Tomokazu Yamazaki, Sangwan Kim, Zhe Yu, Mai Yoshihara, Kohei Takeda, Tsuyoshi Takeshita, Aiko Hirata, Kateřina Bišová, Vilém Zachleder, Masahira Hattori, Shigeyuki Kawano
    • Journal Title

      Biotechnology for Biofuels

      Volume: 9 Issue: 1 Pages: 13-13

    • DOI

      10.1186/s13068-016-0424-2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] What is the correct name for the type species of Haematococcus Flot. (Volvocales, Chlorophyceae)?2016

    • Author(s)
      Nakada,T., Ota, S.
    • Journal Title

      Taxon

      Volume: 65 Issue: 2 Pages: 343-348

    • DOI

      10.12705/652.11

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Morphological changes of giant mitochondria in the unicellular to multicellular phase during parthenogenesis of Ulva partita (Ulvophyceae) revealed by expression of mitochondrial targeting GFP and PEG transformation.2016

    • Author(s)
      Suzuki, R., Ota, S., Yamazaki, T., Toyoda, A., Nonaka, S., Matsukura, C., Kuwano, K., Kawano, S.
    • Journal Title

      Phycological Research

      Volume: 64

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of isolates derived from heavy-ion-beam irradiated cells in the unicellular green alga Parachlorella kessleri2015

    • Author(s)
      Yamazaki, T., Yamashita, Y., Ota, S., Takeshita, T., Kazama, Y., Abe, T., Kawano, S.
    • Journal Title

      RIKEN Accelerator Progress Report

      Volume: 48 Pages: 309-309

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 藻類バイオと電顕3D2015

    • Author(s)
      大田修平、河野重行
    • Journal Title

      Plant Morphology

      Volume: 27 Pages: 3-7

    • NAID

      130005144548

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] <i>Ulva partita</i> sp. nov., a Novel <i>Enteromorpha</i>-Like <i>Ulva</i> Species from Japanese Coastal Areas2015

    • Author(s)
      Kensuke Ichihara, Ryogo Suzuki, Tomokazu Yamazaki, Shuhei Ota, Yuko Mogi, Yayoi Kagami, Kazuyoshi Kuwano, Shigeyuki Kawano
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 3 Pages: 261-270

    • DOI

      10.1508/cytologia.80.261

    • NAID

      130005100897

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Simple Method for Measuring the Starch and Lipid Contents in the Cell of Microalgae2015

    • Author(s)
      Tsuyoshi Takeshita, Kohei Takeda, Shuhei Ota, Tomokazu Yamazaki, Shigeyuki Kawano
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 4 Pages: 475-481

    • DOI

      10.1508/cytologia.80.475

    • NAID

      130005118533

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Life Cycle and Lectin-Binding Patterns in the Chytrid Fungus <i>Chytriomyces hyalinus</i>2015

    • Author(s)
      Ota, S. and Kawano, S.
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 1 Pages: 125-129

    • DOI

      10.1508/cytologia.80.125

    • NAID

      130004801672

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 藻類バイオと電顕 3D2015

    • Author(s)
      大田修平、河野重行
    • Journal Title

      Plant Morphology

      Volume: 27

    • NAID

      130005144548

    • Related Report
      2014 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] 3D-TEM Imaging Demonstrating Dynamic Conversion of Starch and Oil in a Cell of <i>Chlorella sorokiniana</i>2014

    • Author(s)
      Shuhei Ota, Mai Yoshihara, Aiko Hirata, Shigeyuki Kawano
    • Journal Title

      CYTOLOGIA

      Volume: 79 Issue: 3 Pages: 287-288

    • DOI

      10.1508/cytologia.79.287

    • NAID

      130004713088

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Research-status Report
  • [Journal Article] Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae2014

    • Author(s)
      Tomokazu Yamazaki, Chizuru Hirai, Shuhei Ota, Kazuyoshi Kuwano, Shigeyuki Kawano
    • Journal Title

      Cryo Letters

      Volume: 35 Pages: 180-187

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] HAP2/GCS1 Is Involved in the Sexual Reproduction System of the Marine Macroalga<i> Ulva compressa</i> (Ulvales, Chlorophyta)2014

    • Author(s)
      Yamazaki, T., Endo, M., Ito, K., Suzuki, R., Ota, S., Kuwano, K., Miyamura, S., Toyoda, A. and Kawano, S.
    • Journal Title

      CYTOLOGIA

      Volume: 79 Issue: 4 Pages: 575-584

    • DOI

      10.1508/cytologia.79.575

    • NAID

      130004801645

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 微細藻類への重イオンビーム照射によるバイオ燃料増産株の作出2014

    • Author(s)
      松田 尚大、竹下 毅、大田 修平、山﨑 誠和、 風間 裕介、阿部 知子、平田 愛子、河野 重行
    • Journal Title

      生物工学会誌

      Volume: 92 Pages: 602-606

    • Related Report
      2014 Research-status Report
  • [Journal Article] Starch and lipid accumulation in eight strains of six Chlorella species under strong illumination and aeration culture conditions.2014

    • Author(s)
      Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., and Kawano, S.
    • Journal Title

      Bioresour. Technol.

      Volume: 158 Pages: 127-134

    • DOI

      10.1016/j.biortech.2014.01.135

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Image-based monitoring system for green algal Haematococcus pluvialis (Chlorophyceae) cells during culture.2013

    • Author(s)
      Ohnuki S, Nogami S, Ota S, Watanabe K, Kawano S, Ohya Y.
    • Journal Title

      Plant & cell physiology

      Volume: 54(11) Issue: 11 Pages: 1917-29

    • DOI

      10.1093/pcp/pct126

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation.2013

    • Author(s)
      Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S.
    • Journal Title

      Bioresour. Technol.

      Volume: 149 Pages: 432-438

    • DOI

      10.1016/j.biortech.2013.09.079

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of heavy-ion beam irradiation on the survival and growth rates in Parachlorella kessleri2013

    • Author(s)
      Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S.
    • Journal Title

      RIKEN Accel. Prog. Rep.

      Volume: 46 Pages: 266-266

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Haematococcus 属とその近縁属に関する系統分類学的研究2013

    • Author(s)
      大田 修平
    • Journal Title

      藻類 (Jpn. J. Phycol.)

      Volume: 62 Pages: 11-14

    • Related Report
      2013 Research-status Report
  • [Presentation] イオウ飢餓で誘導されるクロレラのオイル蓄積とミトコンドリアと葉緑体のオートファジー2016

    • Author(s)
      大田修平、吉原真衣、山﨑誠和、大島健志朗、服部正平、平田愛子、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      日本歯科大学(東京都千代田区)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ulva partita ゲノムの雌雄特異的領域にある遺伝子とその発現パターン2016

    • Author(s)
      清水恭夫、山﨑誠和、大田修平、市原健介、鈴木亮吾、宮村新一、桑野和可、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      日本歯科大学(東京都千代田区)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硫黄飢餓におけるクロレラの脂質とデンプンの蓄積動態2016

    • Author(s)
      山崎誠和、鴻巣絵梨香、武田行平、竹下毅、平田愛子、大田修平、風間裕介、阿部知子、河野重行
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県、盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] クロレラ6種8株の実験室培養系におけるデンプンとオイルの蓄積と屋外大量培養系への展開2016

    • Author(s)
      竹下毅、大田修平、山崎誠和、河野重行
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県、盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微細緑藻Parachlorella kessleriのシステイン要求性変異体はオイルとデンプンの蓄積動態に異常を示す2015

    • Author(s)
      山﨑誠和、鴻巣絵梨香、竹下毅、大田修平、平田愛子、風間裕介、阿部知子、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島県、鹿児島市)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 強光ストレスによるクロレラ類の劇的な色調変化とカロテノイド蓄積2015

    • Author(s)
      竹下毅、山﨑誠和、大田修平、宮下英明、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島県、鹿児島市)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 栄養飢餓ストレスによるクロレラ類の液胞内ポリリン酸とオートファジーの動態2015

    • Author(s)
      大田修平、吉原真衣、山崎誠和、大島健志朗、許斐麻美、平田愛子、服部正平、 河野重行
    • Organizer
      日本植物学会
    • Place of Presentation
      新潟コンベンションセンター(新潟県、新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 栄養塩飢餓ストレス下のクロレラ類の高電子密度顆粒の微細構造とリンの蓄積動態2015

    • Author(s)
      大田修平、吉原真衣、山﨑誠和、許斐麻美、平田愛子、河野重行
    • Organizer
      日本植物形態学会
    • Place of Presentation
      新潟コンベンションセンター(新潟県、新潟市)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] Parachlorella genome provide insights into sequential accumulation of starch and lipid induced stressfully by sulfur deficiency2015

    • Author(s)
      Shuhei Ota, Tomokazu Yamazaki, Tsuyoshi Takeshita, Aiko Hirata, Katerina Bisova, Vilem Zachleder, Shigeyuki Kawano
    • Organizer
      ヨーロッパ国際藻類学会議
    • Place of Presentation
      イギリス、ロンドン
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chlorella sorokiniana におけるオートファジーの 3 次元微細構造学的研究2015

    • Author(s)
      大田修平、吉原真衣、山﨑誠和、南郷脩史、平田愛子、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      九州大学箱崎キャンパス、福岡市
    • Year and Date
      2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] クロレラの重イオンビーム照射による突然変異誘 導と屋外大量培養株の作出2015

    • Author(s)
      竹下毅、山下雄一、大田 修平、山﨑誠和、大島健志朗、服部正平、風間裕介、阿部知子、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      九州大学箱崎キャンパス、福岡市
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] アカヒゲムシ(Haematococcus;緑藻綱,オオヒゲマワリ目)の異名整理と正名の合意形成に向けて2015

    • Author(s)
      仲田崇志、大田修平
    • Organizer
      日本植物分類学会
    • Place of Presentation
      福島大学金谷川キャンパス、福島市
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 藻類バイオと電顕 3D2014

    • Author(s)
      大田修平、河野重行
    • Organizer
      日本植物学会
    • Place of Presentation
      明治大学生田キャンバス、川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] クロレラ類の栄養飢餓条件における物質の蓄積動態を電顕3D法で立体構築する2014

    • Author(s)
      吉原真衣、大田修平、山﨑誠和、許斐麻美、平田愛子、河野重行
    • Organizer
      日本植物学会
    • Place of Presentation
      明治大学生田キャンバス、川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] クロレラ類のデンプン・オイル蓄積とその生産性に及ぼす強光と屋外大量培養系の影響2014

    • Author(s)
      竹下毅、山﨑誠和、大田修平、Vilem Zachleder 、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      北海道大学、札幌市
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] オイル蓄積を誘導する硫黄飢餓に対する微細緑藻 Parachlorella kessleri のトランスクリプトーム解析2014

    • Author(s)
      山﨑誠和、大田修平、竹下毅、佐藤聖樹、大島健志朗、服部正平、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      北海道大学、札幌市
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Phenotypic spectrum and 3D/TEM analysis of Parachlorella kessleri mutants produced by heavy-ion irradiation2014

    • Author(s)
      Shuhei Ota, Tsuyoshi Takeshita, Tomokazu Yamazaki, Milada Vitova, Vilem Zachleder, Tomoko Abe, Shigeyuki Kawano
    • Organizer
      BioTech 2014 & 6th Czech-Swiss Symposium with Exhibition
    • Place of Presentation
      チェコ共和国、プラハ
    • Year and Date
      2014-06-11 – 2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Starch and lipid accumulation in Chlorella species under high light intensity in the laboratory and outdoor mass cultures2014

    • Author(s)
      Tsuyoshi Takeshita, Ivan Ivanov, Shuhei Ota, Tomokazu Yamazaki, Vilem Zachleder, Shigeyuki Kawano
    • Organizer
      BioTech 2014 & 6th Czech-Swiss Symposium with Exhibition
    • Place of Presentation
      チェコ共和国、プラハ
    • Year and Date
      2014-06-11 – 2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Production of Lipids and Starch in Parachlorella kessleri2014

    • Author(s)
      Ivan Ivanov, Tsuyoshi Takeshita, Shuhie Ota, Tomokazu Yamazaki, Shigeyuki Kawano, Vilem Zachleder, Katerina Bisova
    • Organizer
      BioTech 2014 & 6th Czech-Swiss Symposium with Exhibition
    • Place of Presentation
      チェコ共和国、プラハ
    • Year and Date
      2014-06-11 – 2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Parachlorella kessleri に見られるポリリン酸様高電 子密度顆粒の同定とその蓄積動態

    • Author(s)
      大田修平、吉原真衣、山﨑誠和、仲野靖孝、許斐麻美、平田愛子、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      東邦大学習志野キャンパス、船橋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 強光培養系と屋外大量培養系によるパラクロレラ変異株のデンプンとオイルの蓄積動態の解析

    • Author(s)
      竹下毅、山﨑誠和、大田修平、Vilem Zachleder 、河野重行
    • Organizer
      日本藻類学会
    • Place of Presentation
      東邦大学習志野キャンパス、船橋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 微細藻類への重イオンビーム照射による突然変異率の算出と凍結保存法による変異体の安定性評価

    • Author(s)
      山﨑誠和、大田修平、佐藤聖樹、竹下毅、風間祐介、阿部知子、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島国際会議場、広島市
    • Related Report
      2013 Research-status Report
  • [Presentation] クロレラ6種8株の強光通気培養下のイオウ欠乏に対するオイルとデンプンの蓄積の動態

    • Author(s)
      竹下毅、山﨑誠和、大田修平、河野重行
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島国際会議場、広島市
    • Related Report
      2013 Research-status Report
  • [Presentation] 電顕3Dを用いた栄養塩欠乏ストレスによるクロレラのデンプン・オイル蓄積動態解析

    • Author(s)
      大田修平、吉原真衣、南郷脩史、平田愛子、河野重行
    • Organizer
      日本植物学会
    • Place of Presentation
      北海道大学高等教育推進機構、札幌市
    • Related Report
      2013 Research-status Report
  • [Presentation] 三次元微細構造観察によるクロレラのデンプン・オイル蓄積動態解析

    • Author(s)
      大田修平、吉原真衣、南郷脩史、平田愛子、河野重行
    • Organizer
      日本植物形態学会
    • Place of Presentation
      北海道大学高等教育推進機構、札幌市
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi