Studies on the diversity of the endosymbiotic algae in ciliates

• Project/Area Number: 25840141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: HOSHINA Ryo 長浜バイオ大学, バイオサイエンス学部, 助手 (40373089)
• Research Collaborator: OGURA Atsushi 長浜バイオ大学, バイオサイエンス学部, 准教授 (60465929) ; KUSUOKA Yasushi 滋賀県立琵琶湖博物館, 研究部, 専門学芸員 (80359265) ; SUZAKI Toshinobu 神戸大学, 大学院理学研究科, 准教授 (00187692)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 共生藻 / 植物化 / 継続的共生 / ズークロレラ / 進化 / 細胞内共生 / 繊毛虫 / クロレラ / 共生

Outline of Final Research Achievements

It has been thought that the symbiotic associations between ciliates and their endosymbiotic algae are temporary. We executed continuous sampling of the ciliates and their algal DNA analyses. In consequence, we found that comparatively large number of ciliate species maintain their own algae, and the small number of algal species are shared among different host species. It was found some differences, e.g., nucleotide substitutions in spacer region, in the same species of algae among different host species, which may also indicate the persistent symbioses.

Research Products

• [Journal Article] DNA analysis of algal endosymbionts of ciliates reveals the state of algal integration and the surprising specificity of the symbiosis (2016)
  • Author(s): Ryo Hoshina, Yasushi Kusuoka
  • Journal Title: Protist Volume: 167 Issue: 2 Pages: 174-184
  • DOI: 10.1016/j.protis.2016.02.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Size of Paramecium bursaria individuals under cold and dark conditions (2014)
  • Author(s): Ryo Hoshina
  • Journal Title: Biologia Volume: 69 Issue: 8 Pages: 1018-1022
  • DOI: 10.2478/s11756-014-0404-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA analyses of a private collection of microbial green algae contribute to a better understanding of microbial diversity (2014)
  • Author(s): Ryo Hoshina
  • Journal Title: BMC Research Notes Volume: 7 Issue: 1
  • DOI: 10.1186/1756-0500-7-592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular characterization of Chlorella cultures of the NIES culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., and Didymogenes soliella sp. nov. (Chlorellaceae, Trebouxiophyceae) (2013)
  • Author(s): Ryo Hoshina, Yuko Fujiwara
  • Journal Title: Phycological Research Volume: 61 Issue: 2 Pages: 124-132
  • DOI: 10.1111/pre.12010
  • Peer Reviewed
• [Presentation] A ciliate Cyclotrichium sp. with ocher-colored granules (2016)
  • Author(s): Ryo Hoshina, Toshinabu Suzaki, Yasushi Kusuoka
  • Organizer: Annual meeting International Society of Protistologists/XXI International Society for Evolutionary Protistology
  • Place of Presentation: Moscow, Russia
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] 琵琶湖周辺の共生藻をもつ繊毛虫とその共生藻 II (2016)
  • Author(s): 楠岡泰, 保科 亮
  • Organizer: 琵琶湖博物館研究セミナー
  • Place of Presentation: 草津
  • Year and Date: 2016-03-18
• [Presentation] 琵琶湖から採取された藻類共生繊毛虫 Cyclotrichium spp. について (2016)
  • Author(s): 保科 亮, 楠岡泰
  • Organizer: 日本藻類学会第40回大会
  • Place of Presentation: 東京
  • Year and Date: 2016-03-18
• [Presentation] クロレラ共生種・自由生活種のゲノム比較から迫る共生初期メカニズムの解明 (2015)
  • Author(s): 秋月 祐輝, 保科 亮, 寺林 靖宣, 吉良 聡, 佐藤 昭之, 北川 正成, 小倉 淳
  • Organizer: 日本分子生物学会第38回大会/日本生化学会第88回大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Thinking about the symbiotic state of MARP: the multi-algae retaining protists (2015)
  • Author(s): Ryo Hoshina
  • Organizer: The Second International Simposium of Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2015-09-30
  • Int'l Joint Research / Invited
• [Presentation] シェアされる共生藻 (2015)
  • Author(s): 保科 亮, 早川 昌志、楠岡 泰、洲崎 敏信
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 福岡市
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 琵琶湖周辺の共生藻をもつ繊毛虫とその共生藻 (2015)
  • Author(s): 楠岡 泰, 保科 亮
  • Organizer: 琵琶湖博物館研究セミナー
  • Place of Presentation: 滋賀県草津市
  • Year and Date: 2015-03-20
• [Presentation] The possibility of ‘persistent symbiosis’ in ciliate-algae symbioses (2014)
  • Author(s): Ryo Hoshina, Yasushi Kusuoka
  • Organizer: The second Asian Congress of Protistology/Ninth Asian Conference on Ciliate Biology
  • Place of Presentation: Kalyani, West Bengal, India
  • Year and Date: 2014-11-26 – 2014-11-29
• [Presentation] 琵琶湖産藻類共生繊毛虫にみる共生藻選択 (2014)
  • Author(s): 保科 亮
  • Organizer: 原生生物合同セミナー2014年大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2014-05-24 – 2014-05-25