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Studies on the diversity of the endosymbiotic algae in ciliates

Research Project

Project/Area Number 25840141
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

HOSHINA Ryo  長浜バイオ大学, バイオサイエンス学部, 助手 (40373089)

Research Collaborator OGURA Atsushi  長浜バイオ大学, バイオサイエンス学部, 准教授 (60465929)
KUSUOKA Yasushi  滋賀県立琵琶湖博物館, 研究部, 専門学芸員 (80359265)
SUZAKI Toshinobu  神戸大学, 大学院理学研究科, 准教授 (00187692)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords共生藻 / 植物化 / 継続的共生 / ズークロレラ / 進化 / 細胞内共生 / 繊毛虫 / クロレラ / 共生
Outline of Final Research Achievements

It has been thought that the symbiotic associations between ciliates and their endosymbiotic algae are temporary. We executed continuous sampling of the ciliates and their algal DNA analyses. In consequence, we found that comparatively large number of ciliate species maintain their own algae, and the small number of algal species are shared among different host species. It was found some differences, e.g., nucleotide substitutions in spacer region, in the same species of algae among different host species, which may also indicate the persistent symbioses.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] DNA analysis of algal endosymbionts of ciliates reveals the state of algal integration and the surprising specificity of the symbiosis2016

    • Author(s)
      Ryo Hoshina, Yasushi Kusuoka
    • Journal Title

      Protist

      Volume: 167 Issue: 2 Pages: 174-184

    • DOI

      10.1016/j.protis.2016.02.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Size of Paramecium bursaria individuals under cold and dark conditions2014

    • Author(s)
      Ryo Hoshina
    • Journal Title

      Biologia

      Volume: 69 Issue: 8 Pages: 1018-1022

    • DOI

      10.2478/s11756-014-0404-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA analyses of a private collection of microbial green algae contribute to a better understanding of microbial diversity2014

    • Author(s)
      Ryo Hoshina
    • Journal Title

      BMC Research Notes

      Volume: 7 Issue: 1

    • DOI

      10.1186/1756-0500-7-592

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of Chlorella cultures of the NIES culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., and Didymogenes soliella sp. nov. (Chlorellaceae, Trebouxiophyceae)2013

    • Author(s)
      Ryo Hoshina, Yuko Fujiwara
    • Journal Title

      Phycological Research

      Volume: 61 Issue: 2 Pages: 124-132

    • DOI

      10.1111/pre.12010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] A ciliate Cyclotrichium sp. with ocher-colored granules2016

    • Author(s)
      Ryo Hoshina, Toshinabu Suzaki, Yasushi Kusuoka
    • Organizer
      Annual meeting International Society of Protistologists/XXI International Society for Evolutionary Protistology
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 琵琶湖周辺の共生藻をもつ繊毛虫とその共生藻 II2016

    • Author(s)
      楠岡泰, 保科 亮
    • Organizer
      琵琶湖博物館研究セミナー
    • Place of Presentation
      草津
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 琵琶湖から採取された藻類共生繊毛虫 Cyclotrichium spp. について2016

    • Author(s)
      保科 亮, 楠岡泰
    • Organizer
      日本藻類学会第40回大会
    • Place of Presentation
      東京
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] クロレラ共生種・自由生活種のゲノム比較から迫る共生初期メカニズムの解明2015

    • Author(s)
      秋月 祐輝, 保科 亮, 寺林 靖宣, 吉良 聡, 佐藤 昭之, 北川 正成, 小倉 淳
    • Organizer
      日本分子生物学会第38回大会/日本生化学会第88回大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Thinking about the symbiotic state of MARP: the multi-algae retaining protists2015

    • Author(s)
      Ryo Hoshina
    • Organizer
      The Second International Simposium of Matryoshka-type Evolution of Eukaryotic Cells
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シェアされる共生藻2015

    • Author(s)
      保科 亮, 早川 昌志、楠岡 泰、洲崎 敏信
    • Organizer
      日本藻類学会第39回大会
    • Place of Presentation
      福岡市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 琵琶湖周辺の共生藻をもつ繊毛虫とその共生藻2015

    • Author(s)
      楠岡 泰, 保科 亮
    • Organizer
      琵琶湖博物館研究セミナー
    • Place of Presentation
      滋賀県草津市
    • Year and Date
      2015-03-20
    • Related Report
      2014 Research-status Report
  • [Presentation] The possibility of ‘persistent symbiosis’ in ciliate-algae symbioses2014

    • Author(s)
      Ryo Hoshina, Yasushi Kusuoka
    • Organizer
      The second Asian Congress of Protistology/Ninth Asian Conference on Ciliate Biology
    • Place of Presentation
      Kalyani, West Bengal, India
    • Year and Date
      2014-11-26 – 2014-11-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 琵琶湖産藻類共生繊毛虫にみる共生藻選択2014

    • Author(s)
      保科 亮
    • Organizer
      原生生物合同セミナー2014年大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2014-05-24 – 2014-05-25
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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