| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
A considerable number of species of the Halobacteriaceae possess multiple copies of the 16S rRNA gene that exhibit more than 5% divergence, complicating phylogenetic interpretations. In this study, the family Halobacteriaceae was suggested to split into at least two major groups by the phylogenetic analysis using orthologous 16S rRNA and DNA dependent RNA polymerase subunit H, B” and B’ gene sequences. Furthermore, DNA-DNA hybridization was effective to the genera separation in addition to the species separation in the family Halobacteriaceae. As a result, the genus Halococcus were divided into three groups. Polar membrane lipids analysis of Halococcus species was also supported this result.In the phenotype analysis, Pyruvic acid was almost useful as a nutritional source of haloarchaea. By adding genome analysis data and additional phenotypic data on the data obtained in this study, further refinement of the phylogeny of the Halobacteriaceae is possible.
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