| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The environmental DNA technique was applied to cichlid fish community in Lake Malawi to establish a method for measuring divesity of the community. Sequence data of D-loop and ITS regions were collected from 103 sympatric fish species at the beggining of the study period. Using the sequence data, an eDNA primer targetting D-loop region of cichlids was designed, with which the efficient sampling methods specific to the focal lake was established. Water samples were collected from 12 sites of 6 district of the lake, which differ from each other in cichlid diversity. As interspecific variation in DNA sequence was found to be so poor, I am now trying to design another universal primers that amplify ITS region, with which cichlid diversity will be estimated.
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