| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Reproductive isolation of two closely related bitterlings Tanakia lanceolata and T. limbata, small cyprinid fishes, is known to be incomplete and their hybrids are frequently observed in the wild. To understand the speciation mechanisms of these fishes, phylogenetic and evolutionary analyses were carried out. Phylogenetic analysis by RNA-Seq data suggested that many genes were affected by past hybridization events in these species. Hybrid discrimination analysis using divergent genetic markers showed that frequency of hybridization is highly different among regional populations. F2 hybrids of T. lanceolata and T. limbata have been generated by artificial fertilization. Construction of a linkage map in these F2 hybrids is now in progress.
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