Genetic mechanisms of speciation in two closely related bitterling fishes

• Project/Area Number: 25840145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Osaka Medical College
• Principal Investigator: HASHIGUCHI Yasuyuki 大阪医科大学, 医学部, 講師 (70436517)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 魚類 / タナゴ亜科 / 進化 / 種分化 / 生殖隔離 / RNA-Seq / ゲノム解析 / 適応進化 / RAD-Seq / 生物多様性 / QTL解析 / 多型解析

Outline of Final Research Achievements

Reproductive isolation of two closely related bitterlings Tanakia lanceolata and T. limbata, small cyprinid fishes, is known to be incomplete and their hybrids are frequently observed in the wild. To understand the speciation mechanisms of these fishes, phylogenetic and evolutionary analyses were carried out. Phylogenetic analysis by RNA-Seq data suggested that many genes were affected by past hybridization events in these species. Hybrid discrimination analysis using divergent genetic markers showed that frequency of hybridization is highly different among regional populations. F2 hybrids of T. lanceolata and T. limbata have been generated by artificial fertilization. Construction of a linkage map in these F2 hybrids is now in progress.

Research Products

• [Journal Article] Alteration of development and gene expression induced by in ovo-nanoinjection of 3-hydroxybenzo[c]phenanthrene into Japanese medaka (Oryzias latipes) embryos. (2017)
  • Author(s): Kun Chen, Yuki Tsutsumi, Shuhei Yoshitake, Xuchun Qiu, Hai Xu, Yasuyuki Hashiguchi, Masato Honda, Kosuke Tashiro, Kei Nakayama, Takeshi Hano, Nobuo Suzuki, Kazuichi Hayakawa, Yohei Shimasaki, Yuji Oshima
  • Journal Title: Aquatic Toxicology Volume: 182 Pages: 194-204
  • DOI: 10.1016/j.aquatox.2016.11.020
  • Peer Reviewed
• [Journal Article] Using dense locality sampling resolves the subtle genetic population structure of the dispersive fish species Plecoglossus altivelis. (2016)
  • Author(s): Hirohiko Takeshima, Kei'ichiro Iguchi, Yasuyuki Hashiguchi, Mutsumi Nishida
  • Journal Title: Molecular Ecology Volume: - Issue: 13 Pages: 3048-3064
  • DOI: 10.1111/mec.13650
  • Peer Reviewed / Open Access
• [Journal Article] Characterization and evolutionary analysis of tributyltin-binding protein and pufferfish saxitoxin and tetrodotoxin-binding protein genes in toxic and nontoxic pufferfishes. (2015)
  • Author(s): Hashiguchi Y, Lee JM, Shiraishi M, Komatsu, S., Miki, S., Shimasaki, Y., Mochioka, N., Kusakabe, T., Oshima, Y.
  • Journal Title: Journal of Evolutionary Biology Volume: 28 Issue: 5 Pages: 1103-1118
  • DOI: 10.1111/jeb.12634
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ヤリタナゴ- アブラボテ交雑個体の遺伝的特徴と、種間交雑が各種のゲノムに与える非対称な影響 (2015)
  • Author(s): 橋口康之, 中島淳
  • Organizer: 日本魚類学会
  • Place of Presentation: 近畿大学
  • Year and Date: 2015-09-06
• [Presentation] 魚類におけるCalycinの分子進化～フグ科魚類TBT-bps, PSTBPsを中心に． (2014)
  • Author(s): 橋口康之
  • Organizer: 日本水産学会
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-22
• [Presentation] RNA-Seqデータによるタナゴ亜科魚類の分子系統解析：Tanakia属2種を中心として． (2014)
  • Author(s): 橋口康之
  • Organizer: 日本進化学会
  • Place of Presentation: 高槻現代劇場
  • Year and Date: 2014-08-21 – 2014-08-24
• [Book] Chapter 4: The origin and evolution of the trace amine-associated receptor family in vertebrates. In "Trace amines and neurological disorders: potential mechanisms and risk factors" (2016)
  • Author(s): Yasuyuki Hashiguchi
  • Total Pages: 401
  • Publisher: Elsevier, Academic Press
• [Remarks] NGSデータ解析まとめ サカナ研究者の手探りNGS解析（おもに進化生物学）
  • URL: http://hashiyuki.hatenablog.com
• [Remarks] 研究紹介-Yasuyuki Hashiguchi
  • URL: https://sites.google.com/site/hashiyuki0909/project-definition