Sources of genetic variation for contemporary adaptation to changing environments

• Project/Area Number: 25840161
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Tamagawa University
• Principal Investigator: MIMURA Makiko 玉川大学, 農学部, 准教授 (60451689)
• Research Collaborator: KUNITAKE Hisato 宮崎大学, 農学部, 教授 (80289628) ; IMANISHI Hiroyuki 秋田県立大学, 生物資源科学部, 准教授 (10320607)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: local adaptation / common garden experiment / RADseq / 局所適応 / 相互移植実験 / 分布域 / 気候変動 / ゲノムスキャン / 環境適応 / 適応関連遺伝子 / standing variation / new mutation / introgression

Outline of Final Research Achievements

What makes a species robust to changing environments? In this study, we aimed to identify the sources of genetic variation for contemporary adaptation to past climate changes using common garden experiments and genome-wide RAD-seq analysis in Rubus palmatus. R. palmatus is a wild raspberry species that has a wide species range within Japan and displays a range of phenotypic variations. Its draft genome is available from previous research. In common garden experiments, we found the phonological (bud flush) variation among populations along latitudes at the sampling origins. Genome scan analyses based on genomic SNP data have revealed several SNPs deviated from the neutral expectation, suggesting that they were under natural selection. Some of these SNPs were shared among populations within regions; however, others were unique to only one of two populations studied within regions. These findings imply that standing variation has contributed to rapid adaptation to past climate changes.

Research Products

• [Journal Article] キイチゴたちが屋久島で出会うとき：気候変動と遺伝的交流 (2015)
  • Author(s): 三村真紀子
  • Journal Title: 屋久島学 Volume: 2 Pages: 97-99
• [Journal Article] Range shift and introgression of the rear and leading populations in two ecologically distinct Rubus species (2014)
  • Author(s): Makiko Mimura, Misako Mishima, Martin Lascoux and Tetsukazu Yahara
  • Journal Title: BMC Evolutionary Biology Volume: 14 Issue: 1 Pages: 209-209
  • DOI: 10.1186/s12862-014-0209-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biodiversity only makes sense in the light of evolution (2014)
  • Author(s): R Geeta, Lucia G Lohmann, Susana Magallon, Daniel P Faith, Andrew Hendry, Keith Crandall, Luc de Meester, Cam Webb, Anne-Helene Prieur-Richard, Makiko Mimura, Elena Conti, Joel Cracraft, Felix Forest, Carlos Jaramillo, Michael Donoghue, Tetsukazu Yahara
  • Journal Title: Journal of Biosciences Volume: 39 Issue: 3 Pages: 333-337
  • DOI: 10.1007/s12038-014-9427-y
  • Peer Reviewed
• [Presentation] 標高帯における適応的浸透交雑 (2015)
  • Author(s): 三村真紀子
  • Organizer: 日本生態学会第62回全国大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
  • Year and Date: 2015-03-18 – 2015-03-22
• [Presentation] 気候変動による種の接触と浸透交雑 (2014)
  • Author(s): 三村真紀子・矢原徹一
  • Organizer: 日本生態学会第61回全国大会
  • Place of Presentation: 広島国際会議場（広島市）
• [Presentation] 野生キイチゴのde novo アセンブリ (2014)
  • Author(s): 三村真紀子・山口勝司・重信秀治
  • Organizer: 第 125 回日本森林学会大会
  • Place of Presentation: 大宮ソニックシティ（大宮市）