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Development of a homologous recombination-based plant breeding technique in rice

Research Project

Project/Area Number 25850002
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionNara Institute of Science and Technology

Principal Investigator

SHIMATANI Zenpei  奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (30574701)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords次世代植物育種技術 / イネ / ゲノム編集 / 遺伝子ターゲティング / 形質転換 / ゲノム編集技術 / 新植物育種技術
Outline of Final Research Achievements

Homologous recombination-based gene targeting in rice is a precise and reproducible technique to modify endogenous genes into diverse forms such as knock out, knock-in, creating fusion gene and introducing nucleotide substitution. It is also a promising strategy to develop a “New plant breeding technique (NBT)” if it was combined with a positive selection marker elimination system that leaves no footprints on the targeted gene. In this study, modified plant DNA transposon family was chosen for the system. The codon of a hyper active derivative of the transposase gene was optimized to rice, and its function as a marker gene elimination system was confirmed in rice genome. Then the system was integrated into the gene targeting vector which was designed to introduce two nucleotide substitution to endogenous OsRacGEF1 gene. The modified rice plant is assumed to express constitutive active OsRacGEF1 and show enhanced immune response against to rice blast fungus.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice2015

    • Author(s)
      Shojiro Tamaki, Hiroyuki Tsuji, Ayana Matsumoto, Akiko Fujita, Zenpei Shimatani, Rie Terada, Tomoaki, Sakamoto, Tetsuya Kurata and Ko Shimamoto
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 112 Issue: 8

    • DOI

      10.1073/pnas.1417623112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene editing a constitutively active OsRac1 by homologous recombination-based gene targeting induces immune responses in rice2013

    • Author(s)
      Dang TT, Shimatani Z, Kawano Y, Terada R, Shimamoto K
    • Journal Title

      Plant Cell Physiology

      Volume: 54 Issue: 12 Pages: 2058-2070

    • DOI

      10.1093/pcp/pct147

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 植物ゲノム編集技術としての遺伝子ターゲティング:技術の現状とイネ耐病性関連遺伝子OsRac1の恒常活性化改変2014

    • Author(s)
      島谷 善平, 寺田 理枝 , Dang Thu Thi, 河野 洋治, 辻 寛之, 田岡 健一郎, 島本 功
    • Organizer
      日本育種学会第125回講演会要旨集
    • Place of Presentation
      東北大学
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 植物体に用いる組換えベクター及びその利用2014

    • Inventor(s)
      寺田 理枝, 島谷 善平
    • Industrial Property Rights Holder
      寺田 理枝, 島谷 善平
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-13
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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