| Project/Area Number |
25850003
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMAMOTO Shinji 広島大学, 理学(系)研究科(研究院), 助教 (50607348)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 形質転換 / アグロバクテリア / Tiプラスミド / Riプラスミド |
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| Outline of Final Research Achievements |
In this study, I examined capability of untouched rhizobium strains for a plant transformation technology. Firstly, I established a pathogenic (Ti/Ri) plasmids exchange method, which consists of curing of resident plasmid in recipient cell, addition of transfer origin to the Ti/Ri plasmid in donor cell and conjugation between the donor and the recipient cells. The method was applied to 12 Rhizobium strains for construction of hybrid strains harbor Ti/Ri plasmids from other strains instead of an original one. Total 61 strains including wild type and Ti/Ri-less strains were checked their transformation characteristics, such as an expression of virulence (vir) genes induced by phenolics. As a result, candidate strains useful for transformation, such as the strain strongly expresses vir genes by cocultivation with rice cultured cells, were obtained. The Ti/Ri plasmids exchange method enable us to evaluate the potential characteristics useful for transformation in diverse rhizobium strains.
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