| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Wild type plants of the Japanese morning glory (Ipomoea nil) display bright blue flowers. These flowers contain poly-acylated and -glucosylated anthocyanin pigments, and flower color mutants, dusky and duskish, which lack the glucosylations have been isolated. A flower color mutant dingy accumulates anthocyanins lacking of caffeic acid as the acyl moiety and displays pastel color flowers. It was tried to identified the dingy mutation and clarify the role of the Dingy gene for flower coloration.
At first, cDNA clones for 31 acyltransferase genes were isolated and it was checked whether their gene expressions in the petal were regulated by transcriptional activators for anthocyanin biosynthesis. One candidate acyltrasferase gene, which shows petal-specific expression manner regulated by the transcriptional activators, was characterized. Cloning and structural analysis of the acyltrasferase gene of dingy mutants revealed insertion sequences of transposable elements in two mutant alleles.
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