| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Kobu-sho is a syndrome that causes tumorous or hyperplastic disorders on stems, nodes and roots of gentians. Previously, I identified GK32 gene fragment inducing Kobu-sho-like symptom from Gentian Kobu-sho-associated virus genome. In this study, I aimed to elucidate the mechanism that GK32 protein induced Kobu-sho disease. Gene expression analysis suggested that expression of GK32 gene fragment activated the signaling pathway regulating xylem development. I showed that GK32 protein tagged with yellow fluorescent protein formed many speckles or plate-like structures in the nucleus. GK32 co-localized with Nucleolin (protein localized in nucleolus) and several mRNA splicing factors. I isolated GK32-interacting proteins including many ribosomal proteins using yeast two-hybrid screening.
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