Perturbation of host developmental pathways by virus infection in plant
| Project/Area Number |
25850030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
ATSUMI Go 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究員 (90547217)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 植物ウイルス / 木部 / 木質細胞 / 形態形成 / 二次細胞壁 / 病徴 / 核局在 / 植物ホルモン |
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| Outline of Final Research Achievements |
Kobu-sho is a syndrome that causes tumorous or hyperplastic disorders on stems, nodes and roots of gentians. Previously, I identified GK32 gene fragment inducing Kobu-sho-like symptom from Gentian Kobu-sho-associated virus genome. In this study, I aimed to elucidate the mechanism that GK32 protein induced Kobu-sho disease. Gene expression analysis suggested that expression of GK32 gene fragment activated the signaling pathway regulating xylem development. I showed that GK32 protein tagged with yellow fluorescent protein formed many speckles or plate-like structures in the nucleus. GK32 co-localized with Nucleolin (protein localized in nucleolus) and several mRNA splicing factors. I isolated GK32-interacting proteins including many ribosomal proteins using yeast two-hybrid screening.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] P3N-PIPO of Clover yellow vein virus exacerbates symptoms in pea infected with White clover mosaic virus and is implicated in viral synergism2014
Author(s)
Hisa, Y., Suzuki, H., Atsumi, G., Choi, S.H., Nakahara, K.S., Uyeda, I.
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Journal Title
Virology
Volume: 449
Pages: 200-206
DOI
Related Report
Peer Reviewed
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