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Improvement of salinity tolerance in plants through fine-tuning of the function of the novel Na transporter

Research Project

Project/Area Number 25850041
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionHiroshima University

Principal Investigator

Ueda Akihiro  広島大学, 生物圏科学研究科, 准教授 (10578248)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords塩ストレス / ナトリウム / カリウム / シロイヌナズナ / イネ
Outline of Final Research Achievements

To improve salinity tolerance in plants, functional characterization of NKCC, which is responsible for uptake of sodium, potassium and chroline, was conducted in this study. AtNKCC gene was highly expressed in the root tip regions, and its expression was inducible by potassium deficiency. OsNKCC1, a homolog of AtNKCC in rice, showed the ability for potassium uptake in yeast. Furthermore, a low-Na accumulating rice variety induced expression of the OsNKCC1 gene in response to salinity stress, suggesting that OsNKCC1 participates in potassium uptake in rice. Random mutagenesis was used to understand molecular mechanisms of sodium and potassium transport by AtNKCC. So far, important amino acid residues of AtNKCC for substrate transport have not been identified.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 1 results)

  • [Int'l Joint Research] International Rice Research Institute(フィリピン)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Growth, physiological adaptation, and gene expression analysis of two Egyptian rice cultivars under salt stress2015

    • Author(s)
      Mekawy AMM, Assaha DVM, Yahagi H, Tada Y, Ueda A, Saneoka H
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 87 Pages: 17-25

    • DOI

      10.1016/j.plaphy.2014.12.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Salinity-induced expression of HKT may be crucial for Na+ exclusion in the leaf blade of huckleberry (Solanum scabrum Mill.), but not of eggplant (Solanum melongena L.)2015

    • Author(s)
      Assaha DV, Mekawy AM, Ueda A, Saneoka H
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 460 Issue: 2 Pages: 416-421

    • DOI

      10.1016/j.bbrc.2015.03.048

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and gene expression analysis of ascorbic acid biosynthesis enzymes in Moringa oleifera2015

    • Author(s)
      Kondo T, Fujikawa Y, Ueda A, Nagaoka T, Saneoka H, Martinez M, Calcano M, Martich JDH, Esaka M
    • Journal Title

      African Journal of Agricultural Research

      Volume: 10 Issue: 22 Pages: 2274-2285

    • DOI

      10.5897/ajar2014.9306

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of 5-aminolevulinic acid on Swiss chard (Beta vulgaris L. subsp. cicla) seedling growth under saline conditions2014

    • Author(s)
      Liu L., Nguyen N.T., Ueda A., Saneoka H.
    • Journal Title

      Plant Growth Regulation

      Volume: 74 Issue: 3 Pages: 219-228

    • DOI

      10.1007/s10725-014-9913-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative physiological analysis of salinity tolerance in rice.2013

    • Author(s)
      Ueda A., Yahagi H., Fujikawa Y., Nagaoka T., Esaka M., Calcano M., Martinez M, Martich J.D.H., Saneoka H.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 6 Pages: 896-903

    • DOI

      10.1080/00380768.2013.842883

    • NAID

      110009766790

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of growth and mineral accumulation of two solanaceous species, Solanum scabrum Mill. (huckleberry) and S. melongena L. (eggplant), under salinity stress2013

    • Author(s)
      Assaha D.V.M., Ueda A., Saneoka H.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 6 Pages: 912-920

    • DOI

      10.1080/00380768.2013.858300

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physiological responses of white Swiss chard (Beta vulgaris L. subsp. cicla) to saline and alkaline stresses.2013

    • Author(s)
      Liu L., Ueda A., Saneoka H.
    • Journal Title

      Australian Journal of Crop Science

      Volume: 7 Pages: 1046-1052

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 塩類腺はローズグラスの耐塩性に寄与しているのか?2015

    • Author(s)
      宗廣理子・上田晃弘・実岡寛文
    • Organizer
      2015度日本土壌肥料学会関西支部講演会
    • Place of Presentation
      メルパルク松山
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of a type 3 metallothionein-like gene (OsMT3-a) from rice through functional screening in Escherichia coli, and its overexpression enhanced its tolerance to salinity and heavy-metal stresses2015

    • Author(s)
      Mekawy AMM, Assaha DVM, Munehiro R, Ueda A, Saneoka H
    • Organizer
      2015度日本土壌肥料学会関西支部講演会
    • Place of Presentation
      メルパルク松山
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] The co-expression of SOS1 and HKT genes in Solanum scabrum Mill. is important for reducing Na transport to the leaf blade and in maintaining low Na +/ K + ratios under saline and alkaline stress conditions.2015

    • Author(s)
      Assaha DVM, Akahori T, Mekawy AMM, Ueda A, Saneoka H
    • Organizer
      2015度日本土壌肥料学会関西支部講演会
    • Place of Presentation
      メルパルク松山
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] ローズグラス在来品種群の塩ストレス下における塩類排出特性の解明2015

    • Author(s)
      宗廣理子・上田晃弘・実岡寛文
    • Organizer
      2015年度日本土壌肥料学会東京大会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネの塩ストレス馴化機構の解明2014

    • Author(s)
      松本真由美、上田晃弘、実岡寛文
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 生殖生長期における耐塩性イネ品種の選抜2014

    • Author(s)
      多田祐真、矢萩裕之、上田晃弘、実岡寛文
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Breeding of Low-Phytic Acid Soybean and Characterization of the Novel K/Na Transporter in Arabidopsis Toward Wise Usage of Fertilizer Resources2014

    • Author(s)
      Akihiro Ueda
    • Organizer
      PBGB Division Seminar, International Rice Research Institute
    • Place of Presentation
      Los Banos, Philippines
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Screening of low Na accumulating rice cultivars under salinity stress2013

    • Author(s)
      Yahagi Hiroyuki, Tada Yuma, Mekawy Ahmad M. M., Amas Junrey C., Barretto Aniceta D., Gregorio Glenn B., Ueda Akihiro, Saneoka Hirofumi
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 異なるイネ品種間に見られる塩ストレス応答機構の差異2013

    • Author(s)
      多田祐真、矢萩裕之、上田晃弘、実岡寛文
    • Organizer
      平成25年度日本作物学会中国支部広島大会
    • Place of Presentation
      庄原市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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