Functional analyses of the novel hemoproteins for biomineralizing magnetic particles in magnetotactic bacteria
Project/Area Number |
25850051
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Kanazawa University |
Principal Investigator |
TAOKA Azuma 金沢大学, 自然システム学系, 助教 (20401888)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 細菌 / 磁性 / 磁鉄鉱 / ヘム蛋白質 / シトクロム / バイオミネラリゼーション / 蛋白質 / オルガネラ / 電子伝達 / イメージング / へム蛋白質 / 磁気微粒子 |
Outline of Final Research Achievements |
Magnetotactic bacteria have unique c-type cytochromes for biomineralizing magnetite crystals. We characterized and analyzed one of the magnetotactic bacteria specific cytochrome c MamP using Magnetospirillum magneticum AMB-1. MamP was localized in cytoplasmic membrane, and was specifically expressed during the exponential growth phase. According to MamP overproduction experiments, we found that MamP is crucial to the normal growth of magnetite crystals during the exponential growth phase. Moreover, we showed that MamP can be involved in electron transfer to denitrification pathway, and proposed the new model for the electron transfer pathway during the biomineralization of magnetite.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] 新規巨大磁性細菌の単離
Author(s)
近藤 純也, 田岡 東, 福森 義宏
Organizer
第29回日本微生物生態学会大会
Place of Presentation
鹿児島大学本部キャンパス(鹿児島県鹿児島市)
Related Report
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