| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The novel genes encoding the enzymes (ertB, ertC, ertD) involved in degradation of ergothioneine (ERT) from Burkholderia sp. HME13 were found. The Escherichia coli expression systems of these genes were constructed and only ErtB was expressed as a soluble protein. The novel enzyme, ErtB showed thiolurocanate hydratase activity and the enzyme was characterized. It was showed that ERT was metabolized in glutamate in Burkholderia sp. HME13 cells.
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