Study of the enzymes in the aminolactam metabolic pathway and their utilization for the optically active amino acid synthesis
| Project/Area Number |
25850053
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 微生物変換 / 微生物酵素 / α-アミノラクタム / α-アミノ酸アミド / 加水分解 / ラセミ化 / 加水分解酵素 / ラセマーゼ / 立体選択的反応 / 光学活性物質 |
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| Outline of Final Research Achievements |
α-Aminolactams containing an amino group at the α-position can be considered as cyclic α-amino acid amides. For the study on the metabolism of α-aminolactams, the synthesis of a series of optically active medium-sized α-aminolactams had been already reported by us.
By the screening of microorganisms that can assimilate α-amino-ε-caprolactam (ACL) as a sole source of carbon and nitrogen from soil samples, two microorganisms that can hydrolyze ACL L-enantioselectively and degrade further have been isolated and identified as Mesorhizobium sp. L88 and Aneurinibacillus migulanus L168. This microbial degradation has been developed to the production of D-α-aminolactams under mild conditions. Furthermore, eight bacterial strains that have the ability to racemize ACL have been isolated and identified. Among them, α-aminolactam racemase from Ensifer sp. 23-3. has been purified and characterized.
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Report
(3 results)
Research Products
(4 results)
