| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We examined the side-chain modification of reticuline by introducing glycosyltransferases or sulfotransferases into reticuline-producing system.
In the reticuline-producing strain containing sulfotransferase (SULT1E1) from human, reticuline 3’-O-sulfate was produced from glucose at a yield of 222 mg/L. Furthermore, we showed that UDP-glucuronosyltransferases (UGT2B1) from rat and mouse catalyze the addition of a glucuronic acid moiety to reticuline.
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