The development of plant alkaloid production system by using bacterial platform.
Project/Area Number |
25850054
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Ishikawa Prefectural University |
Principal Investigator |
MINAMI HIROMICHI 石川県立大学, 生物資源環境学部, 准教授 (90433200)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 植物アルカロイド / 発酵 / バイオテクノロジー / 応用微生物 / 酵素反応 |
Outline of Final Research Achievements |
We examined the side-chain modification of reticuline by introducing glycosyltransferases or sulfotransferases into reticuline-producing system. In the reticuline-producing strain containing sulfotransferase (SULT1E1) from human, reticuline 3’-O-sulfate was produced from glucose at a yield of 222 mg/L. Furthermore, we showed that UDP-glucuronosyltransferases (UGT2B1) from rat and mouse catalyze the addition of a glucuronic acid moiety to reticuline.
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Report
(3 results)
Research Products
(9 results)