Identification and Characterization of the Novel Poly(ADP-ribose) glycohydrolase of Filamentous Fungi Aspergillus nidulans

• Project/Area Number: 25850057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Meijo University
• Principal Investigator: SHIMIZU Motoyuki 名城大学, 農学部, 助教 (20423535)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: PARG / Aspergillus / Fungi / poly(ADP-ribose) / PARP / Poly (ADP-ribose) / Aspergillus nidulans / Poly(ADP-ribose)

Outline of Final Research Achievements

Poly(ADP-ribosyl)ation is a post-translational modification of protein in which the ADP-ribose moiety of NAD+ is transferred to amino acids in proteins, altering their structure and function. This protein modification is catalyzed mainly by a nuclear poly(ADP-ribose) polymerase (PARP) in response to several stress, most notably DNA damage. PARP orthologs are found in mammals, plants, metazoans, protists and filamentous fungi, but not in yeast, while PARG homologs are identified in all eukaryotes, besides fungi.
Here, we searched for a parg orthlog in the genome sequence of a model fungus A. nidulans. First, we picked up seven genes as candidates of a fungal parg gene. Proteins coded by the genes were successfully expressed as soluble proteins using Escherichia coli expression system. Among them, only one protein was found to hydrolyze poly(ADP-ribose). This is the first report for identification of PARG in filamentous fungi.

Research Products

• [Journal Article] Achromobacter denitrificans YD35 pyruvate dehydrogenase controls NADH production to tolerate extremely high nitrite levels. (2014)
  • Author(s): Doi, Y, Shimizu, M, Fujita, T, Nakamura, A, Takizawa, N, Takaya, N.
  • Journal Title: Appl. Environ. Microbiol. Volume: 80 Issue: 6 Pages: 1910-1918
  • DOI: 10.1128/aem.03316-13
  • Peer Reviewed / Open Access
• [Journal Article] Nudix Hydrolase Controls Nucleotides and Glycolytic Mechanisms in Hypoxic <i>Aspergillus nidulans</i> (2013)
  • Author(s): Shimizu, M., and Takaya, N.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 9 Pages: 1888-1893
  • DOI: 10.1271/bbb.130334
  • NAID: 10031202740
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] NO-inducible nitrosothionein mediates NO removal in tandem with thioredoxin (2013)
  • Author(s): Zhou S, Narukami T, Masuo S, Shimizu M, Fujita T, Doi Y, Kamimura Y, Takaya N
  • Journal Title: Nat Chem Biol Volume: 9 Issue: 10 Pages: 657-663
  • DOI: 10.1038/nchembio.1316
  • Peer Reviewed
• [Presentation] 糸状菌 Aspergillus nidulans の新規 poly (ADP ribose) glycohydrolase の機能解析とその役割 (2014)
  • Author(s): 宮地雄大,山田麻衣子,平野澪,山本竜也,高谷直樹,志水元亨,加藤雅士
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-09 – 2014-09-12
• [Patent(Industrial Property Rights)] ロイシン酸生産活性を有するタンパク質及びその利用 (2014)
  • Inventor(s): 志水元亨、加藤雅士
  • Industrial Property Rights Holder: 志水元亨、加藤雅士
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-076212
  • Filing Date: 2014-04-02